Curated Information
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Curated Information Page
PubMed Id: 11744698 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Thomas SR, Chen K, Keaney JF (2002) Hydrogen peroxide activates endothelial nitric-oxide synthase through coordinated phosphorylation and dephosphorylation via a phosphoinositide 3-kinase-dependent signaling pathway. J Biol Chem 277, 6017-24 11744698
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

T497-p - eNOS (pig) ▲
Orthologous residues
eNOS (human): T495‑p, eNOS (mouse): T494‑p, eNOS (rat): T494‑p, eNOS (rabbit): T501‑p, eNOS (pig): T497‑p, eNOS (cow): T497‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  PAE (endothelial)
 Cellular systems studied:  cell lines
 Species studied:  pig
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
H2O2 decrease
LY294002 H2O2 inhibit treatment-induced decrease
wortmannin H2O2 inhibit treatment-induced decrease
PP2 H2O2 inhibit treatment-induced decrease
AG1478 H2O2 inhibit treatment-induced decrease
BAPTA-AM H2O2 no effect upon treatment-induced decrease
Ro31-8220 H2O2 no effect upon treatment-induced decrease
LY294002 no change compared to control
wortmannin no change compared to control
Ro31-8220 decrease

S1179-p - eNOS (pig) ▲
Orthologous residues
eNOS (human): S1177‑p, eNOS (mouse): S1176‑p, eNOS (rat): S1176‑p, eNOS (rabbit): S1183‑p, eNOS (pig): S1179‑p, eNOS (cow): S1179‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  PAE (endothelial)
 Cellular systems studied:  cell lines
 Species studied:  pig
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (human) phospho-antibody, transfection of constitutively active enzyme, pharmacological activator of upstream enzyme, pharmacological inhibitor of upstream enzyme, transfection of dominant-negative enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
H2O2 increase
LY294002 H2O2 inhibit treatment-induced increase
wortmannin H2O2 inhibit treatment-induced increase
L-NAME H2O2 no effect upon treatment-induced increase
PP2 H2O2 inhibit treatment-induced increase
AG1478 H2O2 inhibit treatment-induced increase
BAPTA-AM H2O2 inhibit treatment-induced increase
Ro31-8220 H2O2 no effect upon treatment-induced increase
catalase H2O2 inhibit treatment-induced increase


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