Curated Information
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Curated Information Page
PubMed Id: 11788583 
Ku NO, Azhar S, Omary MB (2002) Keratin 8 phosphorylation by p38 kinase regulates cellular keratin filament reorganization: modulation by a keratin 1-like disease causing mutation. J Biol Chem 277, 10775-82 11788583
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
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S74-p - K8 (human)
Orthologous residues
K8 (human): S74‑p, K8 (mouse): S80‑p, K8 (rat): N74‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast), BHK-21 (fibroblast), HT-29 (intestinal)
 Cellular systems studied:  cell lines
 Species studied:  hamster, human, mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE P38A (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE P38A (human) pharmacological inhibitor of upstream enzyme, transfection of wild-type enzyme, transfection of inactive enzyme, co-immunoprecipitation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anisomycin increase
MMS increase
SB203580 anisomycin inhibit treatment-induced increase
PD98059 anisomycin no effect upon treatment-induced increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, protein stabilization
 Effect of modification (process):  cytoskeletal reorganization
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
P38A (human) Induces in vitro, electrophoretic visualization
P38A (human) Induces cytoskeletal reorganization co-immunoprecipitation


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