|
Orthologous residues
|
|
ASK1 (human): S966‑p, ASK1 iso5 (human): S1046‑p, ASK1 (mouse): S973‑p, ASK1 (rat): S930‑p
|
|
Characterization
|
|
Methods used to characterize site in vivo:
[32P] ATP in vitro, immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
|
|
Relevant cell lines - cell types - tissues:
COS (fibroblast)
|
|
Cellular systems studied:
cell lines
|
|
Species studied:
monkey
|
|
Upstream Regulation
|
|
Treatments, proteins and their effect on site modification:
|
|
Treatments
|
Referenced Treatments
|
Manipulated Protein
|
Referenced Protein
|
Effect
|
Notes
|
|
H2O2
|
|
|
|
decrease
|
|
|
NAC
|
H2O2
|
|
|
inhibit treatment-induced decrease
|
|
|
okadaic acid
|
H2O2
|
|
|
inhibit treatment-induced decrease
|
|
|
calyculin A
|
H2O2
|
|
|
inhibit treatment-induced decrease
|
|
|
cyclosporin A
|
H2O2
|
|
|
no effect upon treatment-induced increase
|
|
|
okadaic acid
|
|
|
|
increase
|
|
|
calyculin A
|
|
|
|
increase
|
|
|
cyclosporin A
|
|
|
|
no change compared to control
|
|
|
|
Downstream Regulation
|
|
Effect of modification (function):
enzymatic activity, inhibited, molecular association, regulation
|
|
Modification regulates interactions with:
|
|
Interacting molecule
|
Interacting domains
|
Effect
|
Consequences (function)
|
Consequences (process)
|
Detection assays
|
|
14-3-3 gamma (human)
|
|
Induces
|
molecular association, regulation
|
|
co-immunoprecipitation
|
|
