Curated Information
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Curated Information Page
PubMed Id: 14688258 
Goldman EH, Chen L, Fu H (2004) Activation of apoptosis signal-regulating kinase 1 by reactive oxygen species through dephosphorylation at serine 967 and 14-3-3 dissociation. J Biol Chem 279, 10442-9 14688258
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
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S966-p - ASK1 (human)
Orthologous residues
ASK1 (human): S966‑p, ASK1 iso5 (human): S1046‑p, ASK1 (mouse): S973‑p, ASK1 (rat): S930‑p
Characterization
 Methods used to characterize site in vivo [32P] ATP in vitro, immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
H2O2 decrease
NAC H2O2 inhibit treatment-induced decrease
okadaic acid H2O2 inhibit treatment-induced decrease
calyculin A H2O2 inhibit treatment-induced decrease
cyclosporin A H2O2 no effect upon treatment-induced increase
okadaic acid increase
calyculin A increase
cyclosporin A no change compared to control
Downstream Regulation
 Effect of modification (function):  enzymatic activity, inhibited, molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 gamma (human) Induces molecular association, regulation co-immunoprecipitation


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