Curated Information
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Curated Information Page
PubMed Id: 18644434 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Wang L, Xue J, Zadorozny EV, Robinson LJ (2008) G-CSF stimulates Jak2-dependent Gab2 phosphorylation leading to Erk1/2 activation and cell proliferation. Cell Signal 20, 1890-9 18644434
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y614-p - Gab2 (human)
Orthologous residues
Gab2 (human): Y614‑p, Gab2 (mouse): Y603‑p, Gab2 (rat): Y603‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), DT40GR
 Cellular systems studied:  cell lines
 Species studied:  chicken, human
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SHP-2 (human) Induces co-immunoprecipitation
 Comments:  in the presence of GCSFR;

Y643-p - Gab2 (human)
Orthologous residues
Gab2 (human): Y643‑p, Gab2 (mouse): Y632‑p, Gab2 (rat): Y632‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), DT40GR
 Cellular systems studied:  cell lines
 Species studied:  chicken, human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE JAK2 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JAK2 (human) pharmacological inhibitor of upstream enzyme, antisense inhibition of upstream enzyme, co-immunoprecipitation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
G-CSF increase
AG490 G-CSF inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, phosphorylation
 Effect of modification (process):  cell cycle regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SHP-2 (human) Induces co-immunoprecipitation
 Comments:  activates Erk1/2 and regulates cells proliferation; in the presence of GCSFR;


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