|
Orthologous residues
|
|
NEUROD1 (human): S335‑p, NEUROD1 (mouse): S336‑p, NEUROD1 (rat): S336‑p
|
|
Characterization
|
|
Methods used to characterize site in vivo:
electrophoretic mobility shift, mutation of modification site, phospho-antibody, western blotting
|
|
Relevant cell lines - cell types - tissues:
'neuron, cerebellar granule'-brain
|
|
Cellular systems studied:
primary cultured cells
|
|
Species studied:
rat
|
|
Enzymes shown to modify site in vitro:
|
|
|
|
Upstream Regulation
|
|
Potential in vivo enzymes for site:
|
|
Type
|
Enzyme
|
Evidence
|
Notes
|
|
KINASE
|
CAMK2A (rat)
|
pharmacological inhibitor of upstream enzyme, genetic knockout/knockin of upstream enzyme
|
|
|
|
Treatments, proteins and their effect on site modification:
|
|
Treatments
|
Referenced Treatments
|
Manipulated Protein
|
Referenced Protein
|
Effect
|
Notes
|
|
depolarization
|
|
|
|
increase
|
|
|
KN-62
|
depolarization
|
|
|
inhibit treatment-induced increase
|
|
|
nimodipine
|
depolarization
|
|
|
inhibit treatment-induced increase
|
|
|
U0126
|
depolarization
|
|
|
no effect upon treatment-induced increase
|
|
|
|
Downstream Regulation
|
|
Effect of modification (process):
cell growth, altered
|
