Curated Information
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Curated Information Page
PubMed Id: 18328708 
Praskova M, Xia F, Avruch J (2008) MOBKL1A/MOBKL1B phosphorylation by MST1 and MST2 inhibits cell proliferation. Curr Biol 18, 311-21 18328708
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y15-p - CDK1 (human)
Orthologous residues
CDK1 (human): Y15‑p, CDK1 (mouse): Y15‑p, CDK1 (rat): Y15‑p, CDK1 (chicken): Y15‑p, CDK1 (fruit fly): Y15‑p

S11-p - H3 (human)
Orthologous residues
H3 (human): S11‑p, H3 (mouse): S11‑p, H3 iso2 (mouse): S11‑p, H3 iso3 (mouse): S11‑p, H3 (rat): S11‑p, H3 iso3 (rat): S11‑p, H3 (pig): S11‑p, H3 (chicken): S11‑p, H3 (cow): S11‑p

S909-p - LATS1 (human)
Orthologous residues
LATS1 (human): S909‑p, LATS1 (mouse): S908‑p, LATS1 (rat): S909‑p

T1079-p - LATS1 (human)
Orthologous residues
LATS1 (human): T1079‑p, LATS1 (mouse): T1078‑p, LATS1 (rat): T1079‑p

T12-p - MOB1B (human)
Orthologous residues
MOB1B (human): T12‑p, MOB1B (mouse): T12‑p, MOB1B (rat): T12‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  293 (epithelial), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE MST1 (human)
KINASE MST2 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MST2 (human) co-immunoprecipitation, transfection of inactive enzyme, modification site within consensus motif, siRNA inhibition of enzyme, phospho-antibody, pharmacological activator of upstream enzyme, transfection of wild-type enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
okadaic acid increase
H2O2 increase
nocodazole increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, phosphorylation
 Effect of modification (process):  cell cycle regulation, cell growth, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
LATS2 (human) Induces co-immunoprecipitation
LATS1 (human) Induces co-immunoprecipitation, pull-down assay
NDR2 (human) Induces pull-down assay
NDR1 (human) Induces pull-down assay

T35-p - MOB1B (human)
Orthologous residues
MOB1B (human): T35‑p, MOB1B (mouse): T35‑p, MOB1B (rat): T35‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  293 (epithelial), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE MST1 (human)
KINASE MST2 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MST2 (human) co-immunoprecipitation, transfection of inactive enzyme, modification site within consensus motif, siRNA inhibition of enzyme, phospho-antibody, pharmacological activator of upstream enzyme, transfection of wild-type enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
okadaic acid increase
H2O2 increase
nocodazole increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, phosphorylation
 Effect of modification (process):  cell cycle regulation, cell growth, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
NDR2 (human) Induces pull-down assay
NDR1 (human) Induces pull-down assay
LATS2 (human) Induces co-immunoprecipitation
LATS1 (human) Induces co-immunoprecipitation, pull-down assay

T183-p - MST1 (human)
Orthologous residues
MST1 (human): T183‑p, MST1 (mouse): T183‑p, MST1 (rat): T183‑p

T180-p - MST2 (human)
Orthologous residues
MST2 (human): T180‑p, MST2 (mouse): T180‑p, MST2 (rat): T180‑p


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