Curated Information
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Curated Information Page
PubMed Id: 18614797 
Sarker R, et al. (2008) Casein kinase 2 binds to the C terminus of Na+/H+ exchanger 3 (NHE3) and stimulates NHE3 basal activity by phosphorylating a separate site in NHE3. Mol Biol Cell 19, 3859-70 18614797
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S554-p - NHE3 (rabbit)
Orthologous residues
NHE3 (human): S555‑p, NHE3 (mouse): S550‑p, NHE3 (rat): S552‑p, NHE3 (rabbit): S554‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry
 Relevant cell lines - cell types - tissues:  PS120 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  hamster
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) pharmacological inhibitor of upstream enzyme

S719-p - NHE3 (rabbit)
Orthologous residues
NHE3 (human): S718‑p, NHE3 (mouse): S714‑p, NHE3 (rat): S716‑p, NHE3 (rabbit): S719‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site
 Disease tissue studied:  colorectal cancer, colorectal carcinoma
 Relevant cell lines - cell types - tissues:  Caco-2 (intestinal), PS120 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  hamster
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK2A1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TBB decrease
DMAT decrease
Downstream Regulation
 Effect of modification (function):  activity, induced, intracellular localization


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