Curated Information
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Curated Information Page
PubMed Id: 9312068 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Lu X, Nemoto S, Lin A (1997) Identification of c-Jun NH2-terminal protein kinase (JNK)-activating kinase 2 as an activator of JNK but not p38. J Biol Chem 272, 24751-4 9312068
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T183-p - JNK1 (human)
Orthologous residues
JNK1 (human): T183‑p, JNK1 iso2 (human): T183‑p, JNK1 iso3 (human): T183‑p, JNK1 (mouse): T183‑p, JNK1 (rat): T183‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE MKK7 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MKK7 (human) transfection of wild-type enzyme, activation of upstream enzyme, co-immunoprecipitation
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced
 Effect of modification (process):  transcription, altered

Y185-p - JNK1 (human)
Orthologous residues
JNK1 (human): Y185‑p, JNK1 iso2 (human): Y185‑p, JNK1 iso3 (human): Y185‑p, JNK1 (mouse): Y185‑p, JNK1 (rat): Y185‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE MKK7 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MKK7 (human) transfection of wild-type enzyme, activation of upstream enzyme, co-immunoprecipitation
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced
 Effect of modification (process):  transcription, altered

S271-p - MKK7 (human)
Orthologous residues
MKK7 (human): S271‑p, MKK7 (mouse): S287‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MEKK1 (human) transfection of wild-type enzyme, activation of upstream enzyme, co-immunoprecipitation
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced
 Effect of modification (process):  transcription, altered

T275-p - MKK7 (human)
Orthologous residues
MKK7 (human): T275‑p, MKK7 (mouse): T291‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MEKK1 (human) transfection of wild-type enzyme, activation of upstream enzyme, co-immunoprecipitation
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced
 Effect of modification (process):  transcription, altered


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