Curated Information
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Curated Information Page
PubMed Id: 14985363 
Burchfield JG, et al. (2004) Akt mediates insulin-stimulated phosphorylation of Ndrg2: evidence for cross-talk with protein kinase C theta. J Biol Chem 279, 18623-32 14985363
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S332-p - NDRG2 (mouse)
Orthologous residues
NDRG2 (human): S332‑p, NDRG2 (mouse): S332‑p, NDRG2 (rat): S332‑p
Characterization
 Methods used to characterize site in vivo 2D analysis, [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, phospho-antibody, phosphopeptide mapping, western blotting
 Relevant cell lines - cell types - tissues:  C2C12 (myoblast) [Akt1 (mouse)], C2C12 (myoblast) [NDRG2 (mouse)], C2C12 (myoblast) [PKCT (mouse)]
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKCT (mouse) transfection of wild-type enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
wortmannin insulin inhibit treatment-induced increase

T348-p - NDRG2 (mouse)
Orthologous residues
NDRG2 (human): T348‑p, NDRG2 (mouse): T348‑p, NDRG2 (rat): T348‑p
Characterization
 Methods used to characterize site in vivo 2D analysis, [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, phospho-antibody, phosphopeptide mapping, western blotting
 Relevant cell lines - cell types - tissues:  C2C12 (myoblast) [Akt1 (mouse)], C2C12 (myoblast) [NDRG2 (mouse)], C2C12 (myoblast) [PKCT (mouse)]
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (mouse) transfection of constitutively active enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
wortmannin insulin inhibit treatment-induced increase

S332-p - NDRG2 (rat)
Orthologous residues
NDRG2 (human): S332‑p, NDRG2 (mouse): S332‑p, NDRG2 (rat): S332‑p
Characterization
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCT (mouse)


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