Curated Information
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Curated Information Page
PubMed Id: 14729972 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Davies SL, et al. (2004) Phosphorylation of the Bloom's syndrome helicase and its role in recovery from S-phase arrest. Mol Cell Biol 24, 1279-91 14729972
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T99-p - BLM (human) ▲
Orthologous residues
BLM (human): T99‑p, BLM (mouse): S99‑p
Characterization
 Methods used to characterize site in vivo:  flow cytometry, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  GM9607 (fibroblast), PSNF5 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ATR (human)
KINASE ATM (human)
Downstream Regulation
 Effect of modification (process):  cell cycle regulation
 Comments:  In cells expressing BLM T99A/T122A after HU release, entrance into G1 phase was delayed, with p-Chk-1 levels remaining elevated.

T122-p - BLM (human) ▲
Orthologous residues
BLM (human): T122‑p, BLM (mouse): T122‑p
Characterization
 Methods used to characterize site in vivo:  flow cytometry, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  GM9607 (fibroblast), PSNF5 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ATR (human)
Downstream Regulation
 Effect of modification (process):  cell cycle regulation
 Comments:  In cells expressing BLM T99A/T122A after HU release, entrance into G1 phase was delayed, with p-Chk-1 levels remaining elevated.

S345-p - Chk1 (human) ▲
Orthologous residues
Chk1 (human): S345‑p, Chk1 (mouse): S345‑p, Chk1 (rat): S345‑p, Chk1 (chicken): S345‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  PSNF5 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (process):  cell cycle regulation
 Comments:  In cells expressing BLM T99A/T122A after HU release, entrance into G1 phase was delayed, with p-Chk-1 levels remaining elevated.


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