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Orthologous residues
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AQP2 (human): S256‑p, AQP2 (mouse): S256‑p, AQP2 (rat): S256‑p
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Characterization
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Methods used to characterize site in vivo:
[32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, western blotting
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Relevant cell lines - cell types - tissues:
oocyte [CPEB (mouse)]
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Cellular systems studied:
primary cells
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Species studied:
frog
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Upstream Regulation
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Treatments, proteins and their effect on site modification:
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Treatments
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Referenced Treatments
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Manipulated Protein
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Referenced Protein
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Effect
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Notes
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cAMP analog
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no change compared to control
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H-89
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no change compared to control
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Downstream Regulation
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Effect of modification (function):
intracellular localization, molecular association, regulation
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Modification regulates interactions with:
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Interacting molecule
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Interacting domains
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Effect
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Consequences (function)
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Consequences (process)
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Detection assays
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AQP2 (human)
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Induces
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molecular association, regulation
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electrophoretic visualization
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Comments:
S256-p is found in the plasma membrane in a tetramer, in which 3 out of 4 monomers must be phosphorylated for localization.
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