Curated Information
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Curated Information Page
PubMed Id: 14559997 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Chen MS, Ryan CE, Piwnica-Worms H (2003) Chk1 kinase negatively regulates mitotic function of Cdc25A phosphatase through 14-3-3 binding. Mol Cell Biol 23, 7488-97 14559997
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S178-p - Cdc25A (human)
Orthologous residues
Cdc25A (human): S178‑p, Cdc25A iso2 (human): , Cdc25A (mouse): S172‑p, Cdc25A (rat): S178‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  293 (epithelial), COS (fibroblast), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Chk1 (human)
KINASE Chk2 (human)
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 beta (human) Induces co-immunoprecipitation

T507-p - Cdc25A (human)
Orthologous residues
Cdc25A (human): T507‑p, Cdc25A iso2 (human): T467‑p, Cdc25A (mouse): T497‑p, Cdc25A (rat): T508‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  293 (epithelial), COS (fibroblast), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Chk1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Chk1 (human) inhibition of upstream enzyme Inhibition of Chk1 using siRNA decreased Cdc25A T506 phosphorylation and, to a lesser extent, inhibited S177 phosphorylation.
Downstream Regulation
 Effect of modification (function):  activity, inhibited, molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 beta (human) Induces co-immunoprecipitation
 Comments:  Mutation of T506 to alanine enhanced the activity of Cdc25A, enhancing binding to cyclin B1, activating cyclinB1-Cdk1, and promoting premature entry into mitosis. Binding to 14-3-3 negatively regulates the ability of Cdc25A to dephosphorylate Cdk1.


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