Curated Information
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Curated Information Page
PubMed Id: 12171600 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Knebel A, Haydon CE, Morrice N, Cohen P (2002) Stress-induced regulation of eukaryotic elongation factor 2 kinase by SB 203580-sensitive and -insensitive pathways. Biochem J 367, 525-32 12171600
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T57-p - eEF-2 (human) ▲
Orthologous residues
eEF‑2 (human): T57‑p, eEF‑2 (mouse): T57‑p, eEF‑2 (rat): T57‑p, eEF‑2 (rabbit): T56‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anisomycin decrease
SB203580 anisomycin inhibit treatment-induced decrease
TNF decrease
SB203580 TNF inhibit treatment-induced decrease

S359-p - eEF2K (human) ▲
Orthologous residues
eEF2K (human): S359‑p, eEF2K (mouse): S358‑p, eEF2K (rat): S358‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE p38-delta (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
SB203580 TNF inhibit treatment-induced increase
anisomycin increase
SB203580 anisomycin inhibit treatment-induced increase

S366-p - eEF2K (human) ▲
Orthologous residues
eEF2K (human): S366‑p, eEF2K (mouse): S365‑p, eEF2K (rat): S365‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

S377-p - eEF2K (human) ▲
Orthologous residues
eEF2K (human): S377‑p, eEF2K (mouse): S376‑p, eEF2K (rat): S376‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MAPKAPK2 (human) pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
SB203580 TNF inhibit treatment-induced increase
anisomycin increase
SB203580 anisomycin inhibit treatment-induced increase

S396-p - eEF2K (human) ▲
Orthologous residues
eEF2K (human): S396‑p, eEF2K (mouse): S395‑p, eEF2K (rat): S395‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE p38-beta (human)
KINASE p38-delta (human)


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