Curated Information
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Curated Information Page
PubMed Id: 23671119 
Hughes BT, et al. (2013) Essential role for Cdk2 inhibitory phosphorylation during replication stress revealed by a human Cdk2 knockin mutation. Proc Natl Acad Sci U S A 110, 8954-9 23671119
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T14-p - CDK2 (human)
Orthologous residues
CDK2 (human): T14‑p, CDK2 (mouse): T14‑p, CDK2 (rat): T14‑p
Characterization
 Methods used to characterize site in vivo flow cytometry, immunoprecipitation, mutation of modification site, western blotting
 Disease tissue studied:  bone cancer, colorectal cancer, colorectal carcinoma
 Relevant cell lines - cell types - tissues:  HCT116 (intestinal), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  protein stabilization
 Effect of modification (process):  cell cycle regulation
 Comments:  required for recovery from replication stress; loss of inhibitory phosphorylation causes DNA damage

Y15-p - CDK2 (human)
Orthologous residues
CDK2 (human): Y15‑p, CDK2 (mouse): Y15‑p, CDK2 (rat): Y15‑p
Characterization
 Methods used to characterize site in vivo flow cytometry, immunoprecipitation, mutation of modification site, western blotting
 Disease tissue studied:  bone cancer, colorectal cancer, colorectal carcinoma
 Relevant cell lines - cell types - tissues:  HCT116 (intestinal), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  protein stabilization
 Effect of modification (process):  cell cycle regulation
 Comments:  required for recovery from replication stress; loss of inhibitory phosphorylation causes DNA damage

S140-p - H2AX (human)
Orthologous residues
H2AX (human): S140‑p, H2AX (mouse): S140‑p, H2AX (rat): S140‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  HT116, U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hydroxyurea no change compared to control


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