Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Curated Information Page
PubMed Id: 24197116 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Jiang L, Rong R, Sheikh MS, Huang Y (2014) Mitotic Arrest by Tumor Suppressor RASSF1A Is Regulated via CHK1 Phosphorylation. Mol Cancer Res 12, 119-29 24197116
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

T38-p - RASSF1 iso2 (human)
Orthologous residues
RASSF1 (human): T38‑p, RASSF1 iso2 (human): T38‑p, RASSF1 iso4 (human): , RASSF1 (mouse): T38‑p, RASSF1 iso2 (mouse):
Characterization
 Methods used to characterize site in vivo mass spectrometry (in vitro)
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Chk1 (human)

T43-p - RASSF1 iso2 (human)
Orthologous residues
RASSF1 (human): T43‑p, RASSF1 iso2 (human): T43‑p, RASSF1 iso4 (human): , RASSF1 (mouse): S43‑p, RASSF1 iso2 (mouse):
Characterization
 Methods used to characterize site in vivo mass spectrometry (in vitro)
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Chk1 (human)

S184-p - RASSF1 iso2 (human)
Orthologous residues
RASSF1 (human): S188‑p, RASSF1 iso2 (human): S184‑p, RASSF1 iso4 (human): S114‑p, RASSF1 (mouse): S184‑p, RASSF1 iso2 (mouse): S115‑p
Characterization
 Methods used to characterize site in vivo flow cytometry, mass spectrometry (in vitro), mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Chk1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Chk1 (human) phospho-antibody, transfection of wild-type enzyme, pharmacological inhibitor of upstream enzyme, modification site within consensus motif, transfection of inactive enzyme
Downstream Regulation
 Effect of modification (function):  intracellular localization, molecular association, regulation
 Effect of modification (process):  cell cycle regulation
 Comments:  Phosphorylated RASSF1 regulates association with microtubules and ability to induce mitotic arrest.


Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.