Curated Information
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Curated Information Page
PubMed Id: 12881422 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Morton S, Davis RJ, McLaren A, Cohen P (2003) A reinvestigation of the multisite phosphorylation of the transcription factor c-Jun. EMBO J 22, 3876-86 12881422
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S63-p - Jun (mouse) ▲
Orthologous residues
Jun (human): S63‑p, Jun (mouse): S63‑p, Jun (rat): S63‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast), RAW 264 (macrophage)
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE JNK1 (mouse)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ERK1 (mouse) pharmacological inhibitor of upstream enzyme, phospho-antibody, pharmacological activator of upstream enzyme
KINASE JNK1 (mouse) pharmacological inhibitor of upstream enzyme, phospho-antibody, genetic knockout/knockin of upstream enzyme, pharmacological activator of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS increase
PD184352, SB203580 LPS augment treatment-induced increase 10-15% increase
TNF increase
anisomycin increase
phorbol ester increase
PD184352 phorbol ester inhibit treatment-induced increase
EGF increase
PD184352 EGF inhibit treatment-induced increase

S73-p - Jun (mouse) ▲
Orthologous residues
Jun (human): S73‑p, Jun (mouse): S73‑p, Jun (rat): S73‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast), RAW 264 (macrophage)
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE JNK1 (mouse)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ERK1 (mouse) pharmacological inhibitor of upstream enzyme, phospho-antibody, pharmacological activator of upstream enzyme
KINASE JNK1 (mouse) pharmacological inhibitor of upstream enzyme, phospho-antibody, genetic knockout/knockin of upstream enzyme, pharmacological activator of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS increase
PD184352, SB203580 LPS augment treatment-induced increase 10-15% increase
TNF increase
anisomycin increase
phorbol ester increase
PD184352 phorbol ester inhibit treatment-induced increase
EGF increase
PD184352 EGF inhibit treatment-induced increase

T91-p - Jun (mouse) ▲
Orthologous residues
Jun (human): T91‑p, Jun (mouse): T91‑p, Jun (rat): T91‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast), RAW 264 (macrophage)
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE JNK1 (mouse)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JNK1 (mouse) pharmacological inhibitor of upstream enzyme, phospho-antibody, genetic knockout/knockin of upstream enzyme, pharmacological activator of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS increase
PD184352, SB203580 LPS no effect upon treatment-induced increase
TNF increase
anisomycin increase

T93-p - Jun (mouse) ▲
Orthologous residues
Jun (human): T93‑p, Jun (mouse): T93‑p, Jun (rat): T93‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast), RAW 264 (macrophage)
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE JNK1 (mouse)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JNK1 (mouse) pharmacological inhibitor of upstream enzyme, phospho-antibody, genetic knockout/knockin of upstream enzyme, pharmacological activator of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS increase
PD184352, SB203580 LPS no effect upon treatment-induced increase
TNF increase
anisomycin increase

T242-p - Jun (mouse) ▲
Orthologous residues
Jun (human): T239‑p, Jun (mouse): T242‑p, Jun (rat): T242‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast), RAW 264 (macrophage)
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE GSK3B (mouse)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS decrease
PD184352, wortmannin LPS no effect upon treatment-induced decrease
lithium decrease
EGF decrease
phorbol ester decrease
anisomycin decrease

S246-p - Jun (mouse) ▲
Orthologous residues
Jun (human): S243‑p, Jun (mouse): S246‑p, Jun (rat): S246‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast), RAW 264 (macrophage)
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE GSK3B (mouse)
KINASE ERK2 (mouse)


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