Curated Information
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Curated Information Page
PubMed Id: 12764152 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Woods A, et al. (2003) Identification of phosphorylation sites in AMP-activated protein kinase (AMPK) for upstream AMPK kinases and study of their roles by site-directed mutagenesis. J Biol Chem 278, 28434-42 12764152
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T183-p - AMPKA1 (rat) ▲
Orthologous residues
AMPKA1 (human): T183‑p, AMPKA1 (mouse): T183‑p, AMPKA1 (rat): T183‑p, AMPKA1 (fruit fly): T184‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  CCL13 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  rat
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE LKB1 (mouse)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anoxia increase
osmotic stress increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced

T269-p - AMPKA1 (rat) ▲
Orthologous residues
AMPKA1 (human): T269‑p, AMPKA1 (mouse): A269‑p, AMPKA1 (rat): T269‑p, AMPKA1 (fruit fly): N270‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  CCL13 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  rat
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE LKB1 (mouse)

S496-p - AMPKA1 (rat) ▲
Orthologous residues
AMPKA1 (human): S496‑p, AMPKA1 (mouse): S496‑p, AMPKA1 (rat): S496‑p, AMPKA1 (fruit fly): ‑
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  CCL13 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  rat
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE LKB1 (mouse)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anoxia no change compared to control
osmotic stress decrease

T172-p - AMPKA2 (rat) ▲
Orthologous residues
AMPKA2 (human): T172‑p, AMPKA2 (mouse): T172‑p, AMPKA2 (rat): T172‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  CCL13 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  rat
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE LKB1 (mouse)

T258-p - AMPKA2 (rat) ▲
Orthologous residues
AMPKA2 (human): T258‑p, AMPKA2 (mouse): T258‑p, AMPKA2 (rat): T258‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  CCL13 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  rat
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE LKB1 (mouse)

S491-p - AMPKA2 (rat) ▲
Orthologous residues
AMPKA2 (human): S491‑p, AMPKA2 (mouse): S491‑p, AMPKA2 (rat): S491‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry
 Relevant cell lines - cell types - tissues:  CCL13 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  rat
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE LKB1 (mouse)

S96-p - AMPKB1 (rat) ▲
Orthologous residues
AMPKB1 (human): S96‑p, AMPKB1 (mouse): S96‑p, AMPKB1 (rat): S96‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry
 Relevant cell lines - cell types - tissues:  CCL13 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  rat
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE AMPKA1 (rat)

S101-p - AMPKB1 (rat) ▲
Orthologous residues
AMPKB1 (human): S101‑p, AMPKB1 (mouse): S101‑p, AMPKB1 (rat): S101‑p
Characterization
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE AMPKA1 (rat)

S108-p - AMPKB1 (rat) ▲
Orthologous residues
AMPKB1 (human): S108‑p, AMPKB1 (mouse): S108‑p, AMPKB1 (rat): S108‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry
 Relevant cell lines - cell types - tissues:  CCL13 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  rat
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE AMPKA1 (rat)

S182-p - AMPKB1 (rat) ▲
Orthologous residues
AMPKB1 (human): S182‑p, AMPKB1 (mouse): S182‑p, AMPKB1 (rat): S182‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry
 Relevant cell lines - cell types - tissues:  CCL13 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  rat
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE AMPKA1 (rat)


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