Curated Information
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Curated Information Page
PubMed Id: 9620776 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Waterman MJ, Stavridi ES, Waterman JL, Halazonetis TD (1998) ATM-dependent activation of p53 involves dephosphorylation and association with 14-3-3 proteins. Nat Genet 19, 175-8 9620776
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S376-p - p53 (human) ▲
Orthologous residues
p53 (human): S376‑p, p53 (mouse): S370‑p, p53 iso2 (mouse): ‑, p53 (rat): S374‑p, p53 (rabbit): S374‑p, p53 (monkey): S376‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Disease tissue studied:  bone cancer, breast cancer
 Relevant cell lines - cell types - tissues:  AG1522 (fibroblast), AT5BI, MCF-7 (breast cell), MG87 (fibroblast), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
 Comments:  AT1BR cells
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation decrease
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 zeta (human) Disrupts pull-down assay

S378-p - p53 (human) ▲
Orthologous residues
p53 (human): S378‑p, p53 (mouse): S372‑p, p53 iso2 (mouse): ‑, p53 (rat): S376‑p, p53 (rabbit): S376‑p, p53 (monkey): S378‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Disease tissue studied:  bone cancer, breast cancer
 Relevant cell lines - cell types - tissues:  AG1522 (fibroblast), AT5BI, MCF-7 (breast cell), MG87 (fibroblast), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
 Comments:  AT1BR cells
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE PKACa (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 zeta (human) Induces functional assay, electrophoretic visualization, pull-down assay
DNA Induces electrophoretic visualization


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