Curated Information
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Curated Information Page
PubMed Id: 24213533 
Dunn CA, Lampe PD (2014) Injury-triggered Akt phosphorylation of Cx43: a ZO-1-driven molecular switch that regulates gap junction size. J Cell Sci 127, 455-64 24213533
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T308-p - Akt1 (human)
Orthologous residues
Akt1 (human): T308‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Cellular systems studied:  cell lines
 Species studied:  dog
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
scratch wound increase in human skin increased at 30 min post wounding
deoxygenation increase oxygen reduction to 1%

S473-p - Akt1 (human)
Orthologous residues
Akt1 (human): S473‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Cellular systems studied:  cell lines
 Species studied:  dog
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
scratch wound increase in human skin increased at 30 min post wounding
deoxygenation increase oxygen reduction to 1%

S279-p - GJA1 (human)
Orthologous residues
GJA1 (human): S279‑p, GJA1 (mouse): S279‑p, GJA1 (rat): S279‑p, GJA1 (rabbit): S279‑p, GJA1 (hamster): S279‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Cellular systems studied:  cell lines
 Species studied:  dog
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase rapid phosphorylation of Cx43 at S373 and slower phosphorylation at S279/282

S282-p - GJA1 (human)
Orthologous residues
GJA1 (human): S282‑p, GJA1 (mouse): S282‑p, GJA1 (rat): S282‑p, GJA1 (rabbit): S282‑p, GJA1 (hamster): S282‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Cellular systems studied:  cell lines
 Species studied:  dog
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase rapid phosphorylation of Cx43 at S373 and slower phosphorylation at S279/282

S373-p - GJA1 (human)
Orthologous residues
GJA1 (human): S373‑p, GJA1 (mouse): S373‑p, GJA1 (rat): S373‑p, GJA1 (rabbit): S373‑p, GJA1 (hamster): S373‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MDCK (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  dog
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase rapid phosphorylation of Cx43 at S373 and slower phosphorylation at S279/282
scratch wound increase in human skin increased at 30 min post wounding
deoxygenation increase oxygen reduction to 1%
hypoxia increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
ZO1 (human) Disrupts co-immunoprecipitation
 Comments:  phosphorylated at gap junctions, inhibits or promotes gap junction formation


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