|
Orthologous residues
|
|
CENPA (human): S7‑p, CENPA (mouse): ‑, CENPA (rat): ‑
|
|
Characterization
|
|
Methods used to characterize site in vivo:
microscopy-colocalization with upstream kinase, mutation of modification site, phospho-antibody, western blotting
|
|
Relevant cell lines - cell types - tissues:
HeLa (cervical)
|
|
Cellular systems studied:
cell lines
|
|
Species studied:
human
|
|
Enzymes shown to modify site in vitro:
|
|
|
|
Downstream Regulation
|
|
Effect of modification (function):
molecular association, regulation
|
|
Effect of modification (process):
cell cycle regulation, chromatin organization, altered
|
|
Modification regulates interactions with:
|
|
Interacting molecule
|
Interacting domains
|
Effect
|
Consequences (function)
|
Consequences (process)
|
Detection assays
|
|
AurA (human)
|
|
Induces
|
molecular association, regulation
|
chromatin organization, altered, cell cycle regulation
|
in vitro, co-immunoprecipitation
|
|
|
Comments:
S7A mutation causes defective kinetochore function and mislocalization of Aurora B.
|
