Curated Information
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Curated Information Page
PubMed Id: 24089523 
Williams KA, et al. (2013) Extracellular Signal-regulated Kinase (ERK) Phosphorylates Histone Deacetylase 6 (HDAC6) at Serine 1035 to Stimulate Cell Migration. J Biol Chem 288, 33156-70 24089523
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T1031-p - HDAC6 (human)
Orthologous residues
HDAC6 (human): T1031‑p, HDAC6 (mouse): T971‑p, HDAC6 (rat): T974‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  cervical cancer, cervical adenocarcinoma, lung cancer, non-small cell lung cancer
 Relevant cell lines - cell types - tissues:  293T (epithelial), CHO (fibroblast), HeLa S3 (cervical), MEF (fibroblast), NCI-H1299 (pulmonary)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ERK1 (human) pharmacological inhibitor of upstream enzyme, activation of upstream enzyme, transfection of dominant-negative enzyme, co-immunoprecipitation, siRNA inhibition of enzyme, phospho-antibody
KINASE ERK2 (human) pharmacological inhibitor of upstream enzyme, activation of upstream enzyme, co-immunoprecipitation, siRNA inhibition of enzyme

S1035-p - HDAC6 (human)
Orthologous residues
HDAC6 (human): S1035‑p, HDAC6 (mouse): S975‑p, HDAC6 (rat): S978‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  cervical cancer, cervical adenocarcinoma, lung cancer, non-small cell lung cancer
 Relevant cell lines - cell types - tissues:  293T (epithelial), CHO (fibroblast), HeLa S3 (cervical), MEF (fibroblast), NCI-H1299 (pulmonary)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase
U0126 EGF inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced
 Effect of modification (process):  cell motility, induced


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