Curated Information
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Curated Information Page
PubMed Id: 12842872 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Wilcox HM, Berg LJ (2003) Itk phosphorylation sites are required for functional activity in primary T cells. J Biol Chem 278, 37112-21 12842872
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y186-p - ITK (mouse)
Orthologous residues
ITK (human): Y180‑p, ITK (mouse): Y186‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte), SF9, T lymphocyte-blood
 Cellular systems studied:  cell lines, primary cultured cells
 Species studied:  human, insect, mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ITK (mouse) phospho-antibody, genetic transfer of wild-type enzyme, genetic transfer of dominant-negative enzyme, activation of upstream enzyme
Downstream Regulation
 Effect of modification (function):  phosphorylation
 Comments:  Itk Y186F retains partial activity; stimulates ERK phosphorylation

Y517-p - ITK (mouse)
Orthologous residues
ITK (human): Y512‑p, ITK (mouse): Y517‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte), SF9, T lymphocyte-blood
 Cellular systems studied:  cell lines, primary cultured cells
 Species studied:  human, insect, mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Lck (mouse) phospho-antibody, genetic transfer of wild-type enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-CD3 increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced, phosphorylation
 Comments:  stimulates phosphorylation of ITK Y186

Y783-p - PLCG1 (mouse)
Orthologous residues
PLCG1 (human): Y783‑p, PLCG1 iso2 (human): Y783‑p, PLCG1 (mouse): Y783‑p, PLCG1 (rat): Y783‑p, PLCG1 (cow): Y783‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  SF9
 Cellular systems studied:  cell lines
 Species studied:  fruit fly
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ITK (human) transfection of inactive enzyme


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