Curated Information
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Curated Information Page
PubMed Id: 12855706 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Wu X, Chen J (2003) Autophosphorylation of checkpoint kinase 2 at serine 516 is required for radiation-induced apoptosis. J Biol Chem 278, 36163-8 12855706
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T68-p - Chk2 (human) ▲
Orthologous residues
Chk2 (human): T68‑p, Chk2 iso12 (human): T68‑p, Chk2 (mouse): T77‑p, Chk2 (rat): T76‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, peptide sequencing, phospho-antibody
 Relevant cell lines - cell types - tissues:  293 (epithelial), HCT15 (intestinal), SF9
 Cellular systems studied:  cell lines
 Species studied:  human, insect

S516-p - Chk2 (human) ▲
Orthologous residues
Chk2 (human): S516‑p, Chk2 iso12 (human): S487‑p, Chk2 (mouse): S520‑p, Chk2 (rat): S519‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, peptide sequencing, phospho-antibody
 Relevant cell lines - cell types - tissues:  293 (epithelial), HCT15 (intestinal), SF9
 Cellular systems studied:  cell lines
 Species studied:  human, insect
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Chk2 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Chk2 (human) transfection of wild-type enzyme, transfection of inactive enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced


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