Curated Information
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Curated Information Page
PubMed Id: 18498746 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Hübner A, Barrett T, Flavell RA, Davis RJ (2008) Multisite phosphorylation regulates Bim stability and apoptotic activity. Mol Cell 30, 415-25 18498746
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S55-p - BIM (mouse)
Orthologous residues
BIM (human): S59‑p, BIM iso2 (human): , BIM iso3 (human): , BIM (mouse): S55‑p, BIM (rat): S55‑p
Characterization
 Methods used to characterize site in vivo 2D analysis, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum increase
Downstream Regulation
 Effect of modification (function):  protein degradation
 Effect of modification (process):  apoptosis, inhibited

S65-p - BIM (mouse)
Orthologous residues
BIM (human): S69‑p, BIM iso2 (human): , BIM iso3 (human): , BIM (mouse): S65‑p, BIM (rat): S65‑p
Characterization
 Methods used to characterize site in vivo 2D analysis, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum increase
UV no change compared to control
Downstream Regulation
 Effect of modification (function):  protein degradation
 Effect of modification (process):  apoptosis, inhibited

S73-p - BIM (mouse)
Orthologous residues
BIM (human): S77‑p, BIM iso2 (human): , BIM iso3 (human): , BIM (mouse): S73‑p, BIM (rat): S73‑p
Characterization
 Methods used to characterize site in vivo 2D analysis, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum increase
Downstream Regulation
 Effect of modification (function):  protein degradation
 Effect of modification (process):  apoptosis, inhibited

T112-p - BIM (mouse)
Orthologous residues
BIM (human): T116‑p, BIM iso2 (human): T56‑p, BIM iso3 (human): , BIM (mouse): T112‑p, BIM (rat): T112‑p
Characterization
 Methods used to characterize site in vivo 2D analysis, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  human, mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ERK2 (mouse) phospho-antibody, modification site within consensus motif, pharmacological inhibitor of upstream enzyme, pharmacological activator of upstream enzyme
KINASE ERK1 (mouse) phospho-antibody, modification site within consensus motif, pharmacological inhibitor of upstream enzyme, pharmacological activator of upstream enzyme
KINASE JNK1 (mouse) genetic knockout/knockin of upstream enzyme, transfection of constitutively active enzyme, phospho-antibody, modification site within consensus motif, pharmacological activator of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum increase
U0126 serum inhibit treatment-induced increase
UV increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  apoptosis, induced
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Bcl-2 (mouse) Induces co-immunoprecipitation


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