Curated Information
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Curated Information Page
PubMed Id: 14651849 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Inoki K, Zhu T, Guan KL (2003) TSC2 mediates cellular energy response to control cell growth and survival. Cell 115, 577-90 14651849
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T412-p - p70S6K (human)
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
2-deoxyglucose decrease
siRNA 2-deoxyglucose TSC2 (human) inhibit treatment-induced decrease
FCCP decrease
rottlerin decrease
glucose starvation decrease

T1228-p - TSC2 iso2 (rat)
Orthologous residues
TSC2 (human): T1271‑p, TSC2 iso3 (human): T1227‑p, TSC2 iso4 (human): T1271‑p, TSC2 (mouse): T1270‑p, TSC2 iso6 (mouse): T1271‑p, TSC2 (rat): T1271‑p, TSC2 iso2 (rat): T1228‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE AMPKA1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE AMPKA1 (human) modification site within consensus motif, phosphopeptide analysis, electrophoretic mobility shift, pharmacological activator of upstream enzyme, transfection of constitutively active enzyme, co-immunoprecipitation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
2-deoxyglucose increase
Downstream Regulation
 Effect of modification (function):  phosphorylation
 Comments:  regulates p70S6K phosphorylation

S1338-p - TSC2 iso2 (rat)
Orthologous residues
TSC2 (human): S1379‑p, TSC2 iso3 (human): S1335‑p, TSC2 iso4 (human): S1356‑p, TSC2 (mouse): S1380‑p, TSC2 iso6 (mouse): S1358‑p, TSC2 (rat): S1381‑p, TSC2 iso2 (rat): S1338‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
2-deoxyglucose increase
Downstream Regulation
 Effect of modification (function):  phosphorylation
 Effect of modification (process):  apoptosis, altered, cell growth, altered
 Comments:  regulates p70S6K phosphorylation

S1342-p - TSC2 iso2 (rat)
Orthologous residues
TSC2 (human): S1383‑p, TSC2 iso3 (human): S1339‑p, TSC2 iso4 (human): S1360‑p, TSC2 (mouse): S1384‑p, TSC2 iso6 (mouse): S1362‑p, TSC2 (rat): S1385‑p, TSC2 iso2 (rat): S1342‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
2-deoxyglucose increase
Downstream Regulation
 Effect of modification (function):  phosphorylation
 Effect of modification (process):  apoptosis, altered, cell growth, altered
 Comments:  regulates p70S6K phosphorylation

S1346-p - TSC2 iso2 (rat)
Orthologous residues
TSC2 (human): S1387‑p, TSC2 iso3 (human): S1343‑p, TSC2 iso4 (human): S1364‑p, TSC2 (mouse): S1388‑p, TSC2 iso6 (mouse): S1366‑p, TSC2 (rat): S1389‑p, TSC2 iso2 (rat): S1346‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE AMPKA1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE AMPKA1 (human) modification site within consensus motif, phosphopeptide analysis, electrophoretic mobility shift, pharmacological activator of upstream enzyme, transfection of constitutively active enzyme, co-immunoprecipitation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
2-deoxyglucose increase
Downstream Regulation
 Effect of modification (function):  phosphorylation
 Effect of modification (process):  apoptosis, altered, cell growth, altered
 Comments:  regulates p70S6K phosphorylation


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