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PubMed Id: 16280323 
Shenoy SK, et al. (2006) beta-arrestin-dependent, G protein-independent ERK1/2 activation by the beta2 adrenergic receptor. J Biol Chem 281, 1261-73 16280323
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S345-p - ADRB2 (human)
Orthologous residues
ADRB2 (human): S345‑p, ADRB2 (mouse): S345‑p, ADRB2 (rat): S345‑p, ADRB2 (hamster): S345‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
isoproterenol increase

S346-p - ADRB2 (human)
Orthologous residues
ADRB2 (human): S346‑p, ADRB2 (mouse): S346‑p, ADRB2 (rat): S346‑p, ADRB2 (hamster): S346‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
isoproterenol increase

S355-p - ADRB2 (human)
Orthologous residues
ADRB2 (human): S355‑p, ADRB2 (mouse): S355‑p, ADRB2 (rat): S355‑p, ADRB2 (hamster): S355‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
isoproterenol increase
isoproterenol GRK5 (human) augment treatment-induced increase in T68F,Y132G,Y219A ADRB2 mutant
isoproterenol GRK6 (human) augment treatment-induced increase in T68F,Y132G,Y219A ADRB2 mutant
isoproterenol GRK2 (human) no effect upon treatment-induced increase in T68F,Y132G,Y219A ADRB2 mutant

S356-p - ADRB2 (human)
Orthologous residues
ADRB2 (human): S356‑p, ADRB2 (mouse): S356‑p, ADRB2 (rat): S356‑p, ADRB2 (hamster): S356‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
isoproterenol increase
isoproterenol GRK5 (human) augment treatment-induced increase in T68F,Y132G,Y219A ADRB2 mutant
isoproterenol GRK6 (human) augment treatment-induced increase in T68F,Y132G,Y219A ADRB2 mutant
isoproterenol GRK2 (human) no effect upon treatment-induced increase in T68F,Y132G,Y219A ADRB2 mutant

T202-p - ERK1 (human)
Orthologous residues
ERK1 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
isoproterenol increase
H-89 isoproterenol inhibit treatment-induced increase
PTX isoproterenol inhibit treatment-induced increase
siRNA isoproterenol ARRB1 (human) inhibit treatment-induced increase
siRNA isoproterenol ARRB2 (human) inhibit treatment-induced increase

Y204-p - ERK1 (human)
Orthologous residues
ERK1 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
isoproterenol increase
H-89 isoproterenol inhibit treatment-induced increase
PTX isoproterenol inhibit treatment-induced increase
siRNA isoproterenol ARRB1 (human) inhibit treatment-induced increase
siRNA isoproterenol ARRB2 (human) inhibit treatment-induced increase

T185-p - ERK2 (human)
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
isoproterenol increase
H-89 isoproterenol inhibit treatment-induced increase
PTX isoproterenol inhibit treatment-induced increase
siRNA isoproterenol ARRB1 (human) inhibit treatment-induced increase
siRNA isoproterenol ARRB2 (human) inhibit treatment-induced increase

Y187-p - ERK2 (human)
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
isoproterenol increase
H-89 isoproterenol inhibit treatment-induced increase
PTX isoproterenol inhibit treatment-induced increase
siRNA isoproterenol ARRB1 (human) inhibit treatment-induced increase
siRNA isoproterenol ARRB2 (human) inhibit treatment-induced increase


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