Curated Information
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Curated Information Page
PubMed Id: 18568422 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Horiike Y, Kobayashi H, Sekiguchi T (2009) Ran GTPase guanine nucleotide exchange factor RCC1 is phosphorylated on serine 11 by cdc2 kinase in vitro. Mol Biol Rep 36, 717-23 18568422
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S2-p - RCC1 (human)
Orthologous residues
RCC1 (human): S2‑p, RCC1 iso2 (human): S2‑p, RCC1 (mouse): P2‑p
Characterization
 Methods used to characterize site in vivo 2D analysis, electrophoretic mobility shift, mutation of modification site
 Relevant cell lines - cell types - tissues:  tsBN2 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  hamster

S11-p - RCC1 (human)
Orthologous residues
RCC1 (human): S11‑p, RCC1 iso2 (human): S11‑p, RCC1 (mouse): S11‑p
Characterization
 Methods used to characterize site in vivo 2D analysis, electrophoretic mobility shift, mutation of modification site
 Relevant cell lines - cell types - tissues:  tsBN2 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  hamster
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cytoskeletal reorganization
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
KPNA4 (human) Disrupts co-immunoprecipitation

S20-p - RCC1 (human)
Orthologous residues
RCC1 (human): S20‑p, RCC1 iso2 (human): S20‑p, RCC1 (mouse): S20‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Relevant cell lines - cell types - tissues:  tsBN2 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  hamster
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
KPNA4 (human) Disrupts co-immunoprecipitation

S26-p - RCC1 (human)
Orthologous residues
RCC1 (human): S26‑p, RCC1 iso2 (human): S57‑p, RCC1 (mouse): S26‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Relevant cell lines - cell types - tissues:  tsBN2 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  hamster
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
KPNA4 (human) Disrupts co-immunoprecipitation

S29-p - RCC1 (human)
Orthologous residues
RCC1 (human): S29‑p, RCC1 iso2 (human): S60‑p, RCC1 (mouse): S29‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Relevant cell lines - cell types - tissues:  tsBN2 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  hamster
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
KPNA4 (human) Disrupts co-immunoprecipitation

T32-p - RCC1 (human)
Orthologous residues
RCC1 (human): T32‑p, RCC1 iso2 (human): T63‑p, RCC1 (mouse): T32‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Relevant cell lines - cell types - tissues:  tsBN2 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  hamster
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
KPNA4 (human) Disrupts co-immunoprecipitation

T274-p - RCC1 (human)
Orthologous residues
RCC1 (human): T274‑p, RCC1 iso2 (human): T305‑p, RCC1 (mouse): T274‑p
Characterization
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)

S387-p - RCC1 (human)
Orthologous residues
RCC1 (human): S387‑p, RCC1 iso2 (human): S418‑p, RCC1 (mouse): S387‑p
Characterization
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)


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