Curated Information
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PubMed Id: 18536714 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Winter M, et al. (2008) Control of HIPK2 stability by ubiquitin ligase Siah-1 and checkpoint kinases ATM and ATR. Nat Cell Biol 10, 812-24 18536714
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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K32-u - HIPK2 (human) ▲
Orthologous residues
HIPK2 (human): K32‑u, HIPK2 (mouse): K32‑u, HIPK2 (rat): K32‑u
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting
 Disease tissue studied:  lung cancer, non-small cell lung cancer
 Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  protein degradation

K552-u - HIPK2 (human) ▲
Orthologous residues
HIPK2 (human): K552‑u, HIPK2 (mouse): K552‑u, HIPK2 (rat): K552‑u
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting
 Disease tissue studied:  lung cancer, non-small cell lung cancer
 Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  protein degradation

K565-u - HIPK2 (human) ▲
Orthologous residues
HIPK2 (human): K565‑u, HIPK2 (mouse): K565‑u, HIPK2 (rat): K565‑u
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting
 Disease tissue studied:  lung cancer, non-small cell lung cancer
 Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  protein degradation

K671-u - HIPK2 (human) ▲
Orthologous residues
HIPK2 (human): K671‑u, HIPK2 (mouse): K671‑u, HIPK2 (rat): K671‑u
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting
 Disease tissue studied:  lung cancer, non-small cell lung cancer
 Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  protein degradation

K803-u - HIPK2 (human) ▲
Orthologous residues
HIPK2 (human): K803‑u, HIPK2 (mouse): K803‑u, HIPK2 (rat): K802‑u
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting
 Disease tissue studied:  lung cancer, non-small cell lung cancer
 Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  protein degradation

K805-u - HIPK2 (human) ▲
Orthologous residues
HIPK2 (human): K805‑u, HIPK2 (mouse): K805‑u, HIPK2 (rat): K804‑u
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting
 Disease tissue studied:  lung cancer, non-small cell lung cancer
 Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  protein degradation

K1191-u - HIPK2 (human) ▲
Orthologous residues
HIPK2 (human): K1191‑u, HIPK2 (mouse): K1189‑u, HIPK2 (rat): K1188‑u
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting
 Disease tissue studied:  lung cancer, non-small cell lung cancer
 Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  protein degradation

S46-p - p53 (human) ▲
Orthologous residues
p53 (human): S46‑p, p53 (mouse): L43‑p, p53 iso2 (mouse): L46‑p, p53 (rat): L48‑p, p53 (rabbit): S45‑p, p53 (monkey): S46‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Disease tissue studied:  bone cancer, liver cancer
 Relevant cell lines - cell types - tissues:  HepG2 (hepatic), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
siRNA UV SIAH1 (human) no effect upon treatment-induced increase
siRNA UV MDM2 (human) inhibit treatment-induced increase

S19-p - SIAH1 (human) ▲
Orthologous residues
SIAH1 (human): S19‑p, SIAH1 iso2 (human): S50‑p, SIAH1 (mouse): S19‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  lung cancer, non-small cell lung cancer
 Relevant cell lines - cell types - tissues:  293T (epithelial), NCI-H1299 (pulmonary)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ATM (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) transfection of wild-type enzyme, phospho-antibody, pharmacological inhibitor of upstream enzyme
KINASE ATR (human) transfection of wild-type enzyme, phospho-antibody, pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
caffeine decrease
Downstream Regulation
 Effect of modification (function):  inhibition, molecular association, regulation, ubiquitination
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
HIPK2 (human) Disrupts protein degradation co-immunoprecipitation
 Comments:  facilitates HIPK2 polyubiquitination


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