Curated Information
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Curated Information Page
PubMed Id: 18573880 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Shao J, Welch WJ, Diprospero NA, Diamond MI (2008) Phosphorylation of profilin by ROCK1 regulates polyglutamine aggregation. Mol Cell Biol 28, 5196-208 18573880
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y60-p - profilin 1 (human)
Orthologous residues
profilin 1 (human): Y60‑p, profilin 1 (mouse): F60‑p, profilin 1 (rat): F60‑p, profilin 1 (cow): F60‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), neuron-'brain, cerebral cortex'
 Cellular systems studied:  cell lines, primary cells
 Species studied:  human, rat
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
ACTA1 (human) Induces co-immunoprecipitation

S138-p - profilin 1 (human)
Orthologous residues
profilin 1 (human): S138‑p, profilin 1 (mouse): S138‑p, profilin 1 (rat): S138‑p, profilin 1 (cow): S138‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), neuron-'brain, cerebral cortex'
 Cellular systems studied:  cell lines, primary cells
 Species studied:  human, rat
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE ROCK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ROCK1 (human) phospho-antibody, pharmacological inhibitor of upstream enzyme, pharmacological activator of upstream enzyme, transfection of wild-type enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Y27632 decrease
LPA increase
Y27632 LPA inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  activity, inhibited, molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
ACTA1 (human) Disrupts co-immunoprecipitation
Huntingtin (human) Disrupts co-immunoprecipitation


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