Curated Information
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PubMed Id: 18538787 
Gabant G, et al. (2008) Autophosphorylated residues involved in the regulation of human chk2 in vitro. J Mol Biol 380, 489-503 18538787
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S19-p - Chk2 (human)
Orthologous residues
Chk2 (human): S19‑p, Chk2 iso12 (human): S19‑p, Chk2 (mouse): S22‑p, Chk2 (rat): S21‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  E.coli (bacterial), SF9
 Cellular systems studied:  cell lines
 Species studied:  bacteria, insect
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Chk2 (human)

S33-p - Chk2 (human)
Orthologous residues
Chk2 (human): S33‑p, Chk2 iso12 (human): S33‑p, Chk2 (mouse): S36‑p, Chk2 (rat): S35‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  E.coli (bacterial), SF9
 Cellular systems studied:  cell lines
 Species studied:  bacteria, insect
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Chk2 (human)

S35-p - Chk2 (human)
Orthologous residues
Chk2 (human): S35‑p, Chk2 iso12 (human): S35‑p, Chk2 (mouse): S42‑p, Chk2 (rat): S41‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  E.coli (bacterial), SF9
 Cellular systems studied:  cell lines
 Species studied:  bacteria, insect
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Chk2 (human)

T68-p - Chk2 (human)
Orthologous residues
Chk2 (human): T68‑p, Chk2 iso12 (human): T68‑p, Chk2 (mouse): T77‑p, Chk2 (rat): T76‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  E.coli (bacterial), SF9
 Cellular systems studied:  cell lines
 Species studied:  bacteria, insect

S120-p - Chk2 (human)
Orthologous residues
Chk2 (human): S120‑p, Chk2 iso12 (human): S120‑p, Chk2 (mouse): S124‑p, Chk2 (rat): S123‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  E.coli (bacterial), SF9
 Cellular systems studied:  cell lines
 Species studied:  bacteria, insect
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Chk2 (human)

T225-p - Chk2 (human)
Orthologous residues
Chk2 (human): T225‑p, Chk2 iso12 (human): T225‑p, Chk2 (mouse): T229‑p, Chk2 (rat): T228‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  E.coli (bacterial), SF9
 Cellular systems studied:  cell lines
 Species studied:  bacteria, insect
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Chk2 (human)

S260-p - Chk2 (human)
Orthologous residues
Chk2 (human): S260‑p, Chk2 iso12 (human): S260‑p, Chk2 (mouse): S264‑p, Chk2 (rat): S263‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  E.coli (bacterial), SF9
 Cellular systems studied:  cell lines
 Species studied:  bacteria, insect
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Chk2 (human)

S379-p - Chk2 (human)
Orthologous residues
Chk2 (human): S379‑p, Chk2 iso12 (human): S350‑p, Chk2 (mouse): S383‑p, Chk2 (rat): S382‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  E.coli (bacterial), SF9
 Cellular systems studied:  cell lines
 Species studied:  bacteria, insect
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Chk2 (human)

T387-p - Chk2 (human)
Orthologous residues
Chk2 (human): T387‑p, Chk2 iso12 (human): T358‑p, Chk2 (mouse): T391‑p, Chk2 (rat): T390‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, phospho-antibody, phosphopeptide mapping, western blotting
 Relevant cell lines - cell types - tissues:  E.coli (bacterial), SF9
 Cellular systems studied:  cell lines
 Species studied:  bacteria, insect

T432-p - Chk2 (human)
Orthologous residues
Chk2 (human): T432‑p, Chk2 iso12 (human): T403‑p, Chk2 (mouse): T436‑p, Chk2 (rat): T435‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  E.coli (bacterial)
 Cellular systems studied:  cell lines
 Species studied:  bacteria
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Chk2 (human)

S435-p - Chk2 (human)
Orthologous residues
Chk2 (human): S435‑p, Chk2 iso12 (human): S406‑p, Chk2 (mouse): S439‑p, Chk2 (rat): S438‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  E.coli (bacterial), SF9
 Cellular systems studied:  cell lines
 Species studied:  bacteria, insect
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Chk2 (human)

S516-p - Chk2 (human)
Orthologous residues
Chk2 (human): S516‑p, Chk2 iso12 (human): S487‑p, Chk2 (mouse): S520‑p, Chk2 (rat): S519‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, phospho-antibody, phosphopeptide mapping, western blotting
 Relevant cell lines - cell types - tissues:  E.coli (bacterial), SF9
 Cellular systems studied:  cell lines
 Species studied:  bacteria, insect
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Chk2 (human)


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