Curated Information
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Curated Information Page
PubMed Id: 18458086 
Faisal A, et al. (2008) The Scaffold MyD88 Acts to Couple Protein Kinase C{epsilon} to Toll-like Receptors. J Biol Chem 283, 18591-18600 18458086
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S346-p - PKCE (human)
Orthologous residues
PKCE (human): S346‑p, PKCE (mouse): S346‑p, PKCE (rat): S346‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), macrophage, MEF (fibroblast), RAW 264.7 (macrophage)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS increase
SB415286 LPS inhibit treatment-induced increase
lithium LPS inhibit treatment-induced increase
SB216763 LPS inhibit treatment-induced increase
BIRB-0796 LPS inhibit treatment-induced increase
SB203580 LPS no effect upon treatment-induced increase
SB202190 LPS no effect upon treatment-induced increase
siRNA LPS MYD88 (human) inhibit treatment-induced increase
UV increase
BIRB-0796 UV inhibit treatment-induced increase
SB203580 UV inhibit treatment-induced increase
FSL-1 increase
Pam3CSK4 increase
poly(I-C) no change compared to control
flagellin increase
single-stranded RNA increase
ODN 1826 increase
MYD88 (mouse) increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  transcription, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 beta (human) Induces pull-down assay

S368-p - PKCE (human)
Orthologous residues
PKCE (human): S368‑p, PKCE (mouse): S368‑p, PKCE (rat): S368‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), macrophage, MEF (fibroblast), RAW 264.7 (macrophage)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS increase
bisindolylmaleimide LPS no effect upon treatment-induced increase
Go 6976 LPS inhibit treatment-induced increase
LTA increase
bisindolylmaleimide LTA no effect upon treatment-induced increase
FSL-1 increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  transcription, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 beta (human) Induces pull-down assay


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