Curated Information
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Curated Information Page
PubMed Id: 18451027 
Zhu J, Blenis J, Yuan J (2008) Activation of PI3K/Akt and MAPK pathways regulates Myc-mediated transcription by phosphorylating and promoting the degradation of Mad1. Proc Natl Acad Sci U S A 105, 6584-9 18451027
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
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S145-p - Mad1 (human)
Orthologous residues
Mad1 (human): S145‑p, Mad1 (mouse): S144‑p
Characterization
 Methods used to characterize site in vivo electrophoretic mobility shift, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  breast cancer
 Relevant cell lines - cell types - tissues:  293T (epithelial), HeLa (cervical), MCF-10A (breast cell), MCF-7 (breast cell)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)
KINASE p90RSK (human)
KINASE p70S6K (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE p70S6K (human) inhibition of upstream enzyme, pharmacological activator of upstream enzyme, pharmacological inhibitor of upstream enzyme, siRNA inhibition of enzyme, transfection of constitutively active enzyme, modification site within consensus motif, phospho-antibody, transfection of dominant-negative enzyme, transfection of wild-type enzyme
KINASE p90RSK (human) pharmacological activator of upstream enzyme, pharmacological inhibitor of upstream enzyme, siRNA inhibition of enzyme, modification site within consensus motif, phospho-antibody, transfection of dominant-negative enzyme, transfection of wild-type enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum increase
LY294002 serum no effect upon treatment-induced increase
PD98059 serum no effect upon treatment-induced increase
PD98059, LY294002 serum inhibit treatment-induced increase
rapamycin, PD98059 serum inhibit treatment-induced increase
serum Akt1 (human) inhibit treatment-induced increase dominant negative Akt1
serum p70S6K (human) inhibit treatment-induced increase dominant negative p70S6K
serum p90RSK (human) inhibit treatment-induced increase dominant negative p90RSK
siRNA serum p70S6K (human) no effect upon treatment-induced increase
siRNA serum p90RSK (human) no effect upon treatment-induced increase
siRNA serum p70S6K (human), p90RSK (human) inhibit treatment-induced increase
insulin increase
LY294002 insulin no effect upon treatment-induced increase
PD98059 insulin no effect upon treatment-induced increase
PD98059, LY294002 insulin inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  protein degradation
 Effect of modification (process):  cell growth, altered, transcription, induced


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