Curated Information
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Curated Information Page
PubMed Id: 12697768 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Ward IM, Minn K, Jorda KG, Chen J (2003) Accumulation of checkpoint protein 53BP1 at DNA breaks involves its binding to phosphorylated histone H2AX. J Biol Chem 278, 19579-82 12697768
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S6-p - 53BP1 (human)
Orthologous residues
53BP1 (human): S6‑p, 53BP1 (mouse): S6‑p, 53BP1 (rat): S11‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  ES (stem), U2OS (bone cell) [GR (human)]
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase

S25-p - 53BP1 (human)
Orthologous residues
53BP1 (human): S25‑p, 53BP1 (mouse): S25‑p, 53BP1 (rat): S30‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  ES (stem), U2OS (bone cell) [GR (human)]
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) genetic knockout/knockin of upstream enzyme, pharmacological activator of upstream enzyme, modification site within consensus motif, phospho-antibody
 Comments:  Only checked in vivo with pS25/S29 antibody. S6 and S784 not tested.
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase

S29-p - 53BP1 (human)
Orthologous residues
53BP1 (human): S29‑p, 53BP1 (mouse): S29‑p, 53BP1 (rat): S34‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  ES (stem), U2OS (bone cell) [GR (human)]
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) genetic knockout/knockin of upstream enzyme, pharmacological activator of upstream enzyme, modification site within consensus motif, phospho-antibody
 Comments:  Only checked in vivo with pS25/S29 antibody. S6 and S784 not tested.
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase

S784-p - 53BP1 (human)
Orthologous residues
53BP1 (human): S784‑p, 53BP1 (mouse): S770‑p, 53BP1 (rat): S785‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  ES (stem), U2OS (bone cell) [GR (human)]
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase

S140-p - H2AX (human)
Orthologous residues
H2AX (human): S140‑p, H2AX (mouse): S140‑p, H2AX (rat): S140‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  ES (stem), U2OS (bone cell) [GR (human)]
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
53BP1 (human) Induces intracellular localization pull-down assay


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