Curated Information
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PubMed Id: 12697768 
Ward IM, Minn K, Jorda KG, Chen J (2003) Accumulation of checkpoint protein 53BP1 at DNA breaks involves its binding to phosphorylated histone H2AX. J Biol Chem 278, 19579-82 12697768
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
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S6-p - 53BP1 (human)
Orthologous residues
53BP1 (human): S6‑p, 53BP1 iso2 (human): S11‑p, 53BP1 iso3 (human): S11‑p, 53BP1 (mouse): S6‑p, 53BP1 (rat): S11‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  ES (stem), U2OS (bone cell) [GR (human)]
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase

S25-p - 53BP1 (human)
Orthologous residues
53BP1 (human): S25‑p, 53BP1 iso2 (human): S30‑p, 53BP1 iso3 (human): S30‑p, 53BP1 (mouse): S25‑p, 53BP1 (rat): S30‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  ES (stem), U2OS (bone cell) [GR (human)]
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) pharmacological activator of upstream enzyme, modification site within consensus motif, phospho-antibody, genetic knockout/knockin of upstream enzyme
 Comments:  Only checked in vivo with pS25/S29 antibody. S6 and S784 not tested.
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase

S29-p - 53BP1 (human)
Orthologous residues
53BP1 (human): S29‑p, 53BP1 iso2 (human): S34‑p, 53BP1 iso3 (human): S34‑p, 53BP1 (mouse): S29‑p, 53BP1 (rat): S34‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  ES (stem), U2OS (bone cell) [GR (human)]
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) pharmacological activator of upstream enzyme, modification site within consensus motif, phospho-antibody, genetic knockout/knockin of upstream enzyme
 Comments:  Only checked in vivo with pS25/S29 antibody. S6 and S784 not tested.
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase

S784-p - 53BP1 (human)
Orthologous residues
53BP1 (human): S784‑p, 53BP1 iso2 (human): S789‑p, 53BP1 iso3 (human): S789‑p, 53BP1 (mouse): S770‑p, 53BP1 (rat): S785‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  ES (stem), U2OS (bone cell) [GR (human)]
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase

S139-p - H2AX (human)
Orthologous residues
H2AX (human): S139‑p, H2AX (mouse): S139‑p, H2AX (rat): S139‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  ES (stem), U2OS (bone cell) [GR (human)]
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
53BP1 (human) Induces intracellular localization pull-down assay


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