Curated Information
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PubMed Id: 11044453 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Dans M, et al. (2001) Tyrosine phosphorylation of the beta 4 integrin cytoplasmic domain mediates Shc signaling to extracellular signal-regulated kinase and antagonizes formation of hemidesmosomes. J Biol Chem 276, 1494-502 11044453
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y1422-p - ITGB4 iso2 (human) ▲
Orthologous residues
ITGB4 (human): Y1492‑p, ITGB4 iso2 (human): Y1422‑p, ITGB4 iso3 (human): Y1422‑p, ITGB4 (mouse): Y1488‑p, ITGB4 iso2 (mouse): Y1423‑p, ITGB4 iso3 (mouse): Y1423‑p, ITGB4 (rat): Y1423‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  bladder cancer
 Relevant cell lines - cell types - tissues:  293T (epithelial), 804G (epithelial), HaCaT (keratinocyte), HUVEC (endothelial)
 Cellular systems studied:  cell lines
 Species studied:  human, rat
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cell adhesion, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Shc1 (human) SH2 Induces far-Western, sequence-specific competitor

Y1440-p - ITGB4 iso2 (human) ▲
Orthologous residues
ITGB4 (human): Y1510‑p, ITGB4 iso2 (human): Y1440‑p, ITGB4 iso3 (human): Y1440‑p, ITGB4 (mouse): Y1506‑p, ITGB4 iso2 (mouse): Y1441‑p, ITGB4 iso3 (mouse): Y1441‑p, ITGB4 (rat): Y1441‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  bladder cancer
 Relevant cell lines - cell types - tissues:  293T (epithelial), 804G (epithelial), HaCaT (keratinocyte), HUVEC (endothelial)
 Cellular systems studied:  cell lines
 Species studied:  human, rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
vanadate increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cell adhesion, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Shc1 (human) SH2 Induces far-Western, sequence-specific competitor

Y1526-p - ITGB4 iso2 (human) ▲
Orthologous residues
ITGB4 (human): Y1596‑p, ITGB4 iso2 (human): Y1526‑p, ITGB4 iso3 (human): Y1579‑p, ITGB4 (mouse): Y1592‑p, ITGB4 iso2 (mouse): Y1527‑p, ITGB4 iso3 (mouse): Y1580‑p, ITGB4 (rat): Y1580‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  bladder cancer
 Relevant cell lines - cell types - tissues:  293T (epithelial), 804G (epithelial), HaCaT (keratinocyte), HUVEC (endothelial)
 Cellular systems studied:  cell lines
 Species studied:  human, rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
vanadate increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cell adhesion, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Shc1 (human) PTB Induces activation, phosphorylation far-Western, sequence-specific competitor

Y1642-p - ITGB4 iso2 (human) ▲
Orthologous residues
ITGB4 (human): Y1712‑p, ITGB4 iso2 (human): Y1642‑p, ITGB4 iso3 (human): Y1695‑p, ITGB4 (mouse): Y1708‑p, ITGB4 iso2 (mouse): Y1643‑p, ITGB4 iso3 (mouse): Y1696‑p, ITGB4 (rat): Y1696‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  bladder cancer
 Relevant cell lines - cell types - tissues:  293T (epithelial), 804G (epithelial), HaCaT (keratinocyte), HUVEC (endothelial)
 Cellular systems studied:  cell lines
 Species studied:  human, rat
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cell adhesion, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Shc1 (human) PTB Induces far-Western, sequence-specific competitor


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