Curated Information
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Curated Information Page
PubMed Id: 23429703 
Ben-Sahra I, Howell JJ, Asara JM, Manning BD (2013) Stimulation of de novo pyrimidine synthesis by growth signaling through mTOR and S6K1. Science 339, 1323-8 23429703
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
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S1859-p - CAD (human)
Orthologous residues
CAD (human): S1859‑p, CAD (mouse): S1859‑p, CAD iso2 (mouse): S132‑p, CAD (rat): S1859‑p, CAD (hamster): S1859‑p
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical)
 Cellular systems studied:  cell lines
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE p70S6K (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE p70S6K (human) pharmacological inhibitor of upstream enzyme, siRNA inhibition of enzyme, co-immunoprecipitation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
rapamycin insulin inhibit treatment-induced increase
PF-4708671 insulin inhibit treatment-induced increase
siRNA insulin inhibit treatment-induced increase S6K1 siRNA
EGF increase
rapamycin EGF inhibit treatment-induced increase
PF-4708671 EGF inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced

S1900-p - CAD (human)
Orthologous residues
CAD (human): S1900‑p, CAD (mouse): S1900‑p, CAD iso2 (mouse): S190‑p, CAD (rat): S1900‑p, CAD (hamster): S1900‑p
 Methods used to characterize site in vivo mass spectrometry

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