Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Curated Information Page
PubMed Id: 23429703 
Ben-Sahra I, Howell JJ, Asara JM, Manning BD (2013) Stimulation of de novo pyrimidine synthesis by growth signaling through mTOR and S6K1. Science 339, 1323-8 23429703
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

S1859-p - CAD (human)
Orthologous residues
CAD (human): S1859‑p, CAD (mouse): S1859‑p, CAD iso2 (mouse): S132‑p, CAD (rat): S1859‑p, CAD (hamster): S1859‑p
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical)
 Cellular systems studied:  cell lines
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE p70S6K (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE p70S6K (human) co-immunoprecipitation, pharmacological inhibitor of upstream enzyme, siRNA inhibition of enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
rapamycin insulin inhibit treatment-induced increase
PF-4708671 insulin inhibit treatment-induced increase
siRNA insulin inhibit treatment-induced increase S6K1 siRNA
EGF increase
rapamycin EGF inhibit treatment-induced increase
PF-4708671 EGF inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced

S1900-p - CAD (human)
Orthologous residues
CAD (human): S1900‑p, CAD (mouse): S1900‑p, CAD iso2 (mouse): S190‑p, CAD (rat): S1900‑p, CAD (hamster): S1900‑p
 Methods used to characterize site in vivo mass spectrometry

Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.