Curated Information
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Curated Information Page
PubMed Id: 11160134 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Rui L, et al. (2001) Insulin/IGF-1 and TNF-alpha stimulate phosphorylation of IRS-1 at inhibitory Ser307 via distinct pathways. J Clin Invest 107, 181-9 11160134
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S312-p - IRS1 (human) ▲
Orthologous residues
IRS1 (human): S312‑p, IRS1 (mouse): S307‑p, IRS1 (rat): S307‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal'
 Cellular systems studied:  tissue
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase

S307-p - IRS1 (mouse) ▲
Orthologous residues
IRS1 (human): S312‑p, IRS1 (mouse): S307‑p, IRS1 (rat): S307‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal'
 Cellular systems studied:  tissue
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase

S307-p - IRS1 (rat) ▲
Orthologous residues
IRS1 (human): S312‑p, IRS1 (mouse): S307‑p, IRS1 (rat): S307‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, skeletal', 293 (epithelial), 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], CHO (fibroblast) [EphB1 (human), transfection]
 Cellular systems studied:  cell lines, tissue
 Species studied:  hamster, human, mouse, rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anisomycin increase
insulin increase
LY294002 insulin inhibit treatment-induced increase
wortmannin insulin inhibit treatment-induced increase
PD98059 insulin no effect upon treatment-induced increase
IGF-1 increase
LY294002 IGF-1 inhibit treatment-induced increase
wortmannin IGF-1 inhibit treatment-induced increase
PD98059 IGF-1 no effect upon treatment-induced increase
TNF increase
LY294002 TNF no effect upon treatment-induced increase
wortmannin TNF no effect upon treatment-induced increase
PD98059 TNF inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  phosphorylation


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