Curated Information
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Curated Information Page
PubMed Id: 17386261 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Larochelle S, et al. (2007) Requirements for Cdk7 in the assembly of Cdk1/cyclin B and activation of Cdk2 revealed by chemical genetics in human cells. Mol Cell 25, 839-50 17386261
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T161-p - CDK1 (human)
Orthologous residues
CDK1 (human): T161‑p, CDK1 (mouse): T161‑p, CDK1 (rat): T161‑p, CDK1 (chicken): T161‑p, CDK1 (fruit fly): T161‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  colorectal cancer, colorectal carcinoma
 Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  wt and CDK7(as/as) HCT116
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
CDK7 (human) decrease inhibition of CDK7(as) allele via 1-NMPP1
serum starvation decrease
actinomycin D increase
actinomycin D CDK7 (human) inhibit treatment-induced increase inhibition of CDK7(as) allele via 1-NMPP1

T160-p - CDK2 (human)
Orthologous residues
CDK2 (human): T160‑p, CDK2 (mouse): T160‑p, CDK2 (rat): T160‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  colorectal cancer, colorectal carcinoma
 Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  wt and CDK7(as/as) HCT116
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
CDK7 (human) decrease inhibition of CDK7(as) allele via 1-NMPP1
serum increase
serum CDK7 (human) inhibit treatment-induced increase inhibition of CDK7(as) allele via 1-NMPP1

S1619-p - POLR2A (human)
Orthologous residues
POLR2A (human): S1619‑p, POLR2A var1 (human): S1619‑p, POLR2A (mouse): S1619‑p, POLR2A (rat): S1619‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  colorectal cancer, colorectal carcinoma
 Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  wt and CDK7(as/as) HCT116
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
CDK7 (human) increase inhibition of CDK7(as) allele via 1-NMPP1


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