Arabi A, et al. (2012) Proteomic screen reveals Fbw7 as a modulator of the NF-κB pathway. Nat Commun 3, 976 22864569

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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S707-p - NFkB-p100 (human) ▲

Modsite: EPLCPLPsPPTsDsD SwissProt Entrez-Gene Orthologous residues NFkB‑p100 (human): S707‑p, NFkB‑p100 iso3 (human): ‑, NFkB‑p100 iso4 (human): S707‑p, NFkB‑p100 (mouse): S707‑p, NFkB‑p100 (rat): S706‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, western blotting Disease tissue studied:  colorectal cancer, colorectal carcinoma Relevant cell lines - cell types - tissues:  293 (epithelial), HCT116 (intestinal) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE GSK3B (human) modification site within consensus motif, pharmacological inhibitor of upstream enzyme Downstream Regulation Effect of modification (function):  protein degradation, ubiquitination Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays FBXW7 (human) Induces co-immunoprecipitation

S711-p - NFkB-p100 (human) ▲

Modsite: PLPsPPTsDsDsDsE SwissProt Entrez-Gene Orthologous residues NFkB‑p100 (human): S711‑p, NFkB‑p100 iso3 (human): ‑, NFkB‑p100 iso4 (human): S711‑p, NFkB‑p100 (mouse): S711‑p, NFkB‑p100 (rat): S710‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, western blotting Disease tissue studied:  colorectal cancer, colorectal carcinoma Relevant cell lines - cell types - tissues:  293 (epithelial), HCT116 (intestinal) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE GSK3B (human) modification site within consensus motif, pharmacological inhibitor of upstream enzyme Downstream Regulation Effect of modification (function):  protein degradation, ubiquitination Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays FBXW7 (human) Induces co-immunoprecipitation