Curated Information
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Curated Information Page
PubMed Id: 22864569 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Arabi A, et al. (2012) Proteomic screen reveals Fbw7 as a modulator of the NF-κB pathway. Nat Commun 3, 976 22864569
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S707-p - NFkB-p100 (human) ▲
Orthologous residues
NFkB‑p100 (human): S707‑p, NFkB‑p100 (mouse): S707‑p, NFkB‑p100 (rat): S706‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, western blotting
 Disease tissue studied:  colorectal cancer, colorectal carcinoma
 Relevant cell lines - cell types - tissues:  293 (epithelial), HCT116 (intestinal)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) modification site within consensus motif, pharmacological inhibitor of upstream enzyme
Downstream Regulation
 Effect of modification (function):  protein degradation, ubiquitination
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
FBXW7 (human) Induces co-immunoprecipitation

S711-p - NFkB-p100 (human) ▲
Orthologous residues
NFkB‑p100 (human): S711‑p, NFkB‑p100 (mouse): S711‑p, NFkB‑p100 (rat): S710‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, western blotting
 Disease tissue studied:  colorectal cancer, colorectal carcinoma
 Relevant cell lines - cell types - tissues:  293 (epithelial), HCT116 (intestinal)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) modification site within consensus motif, pharmacological inhibitor of upstream enzyme
Downstream Regulation
 Effect of modification (function):  protein degradation, ubiquitination
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
FBXW7 (human) Induces co-immunoprecipitation


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