Curated Information
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Curated Information Page
PubMed Id: 18400164 
Ma YC, et al. (2008) Regulation of motor neuron specification by phosphorylation of neurogenin 2. Neuron 58, 65-77 18400164
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S231-p - neurogenin 2 (mouse)
Orthologous residues
neurogenin 2 (human): S239‑p, neurogenin 2 (mouse): S231‑p, neurogenin 2 (rat): S231‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'brain, embryonic', 'stem, embryonic', 293T (epithelial)
 Cellular systems studied:  cell lines, primary cultured cells, tissue
 Species studied:  human, mouse
 Comments:  E11.5 neural tube and telencephalon
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE GSK3B (human)
KINASE GSK3A (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (mouse) pharmacological inhibitor of upstream enzyme, siRNA inhibition of enzyme
KINASE GSK3A (mouse) pharmacological inhibitor of upstream enzyme, siRNA inhibition of enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
GSK-3 inhibitor X decrease
Shh increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cell growth, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
LDB1 (mouse) Induces co-immunoprecipitation
 Comments:  important for spinal motor neuron differentiation

S234-p - neurogenin 2 (mouse)
Orthologous residues
neurogenin 2 (human): S242‑p, neurogenin 2 (mouse): S234‑p, neurogenin 2 (rat): S234‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'brain, embryonic', 'stem, embryonic', 293T (epithelial)
 Cellular systems studied:  cell lines, primary cultured cells, tissue
 Species studied:  human, mouse
 Comments:  E11.5 neural tube and telencephalon
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE GSK3A (human)
KINASE GSK3B (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (mouse) pharmacological inhibitor of upstream enzyme, siRNA inhibition of enzyme
KINASE GSK3A (mouse) pharmacological inhibitor of upstream enzyme, siRNA inhibition of enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
GSK-3 inhibitor X decrease
Shh increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cell growth, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
LDB1 (mouse) Induces co-immunoprecipitation
 Comments:  important for spinal motor neuron differentiation


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