|
Orthologous residues
|
|
PDK1 (human): S241‑p, PDK1 iso4 (human): S114‑p, PDK1 (mouse): S244‑p, PDK1 (rat): S244‑p
|
|
Characterization
|
|
Methods used to characterize site in vivo:
mutation of modification site
|
|
Disease tissue studied:
fibrosarcoma of soft tissue
|
|
Relevant cell lines - cell types - tissues:
293 (epithelial), COS (fibroblast), HT1080 (fibroblast)
|
|
Cellular systems studied:
cell lines
|
|
Species studied:
human, monkey
|
|
Enzymes shown to modify site in vitro:
|
|
|
|
Downstream Regulation
|
|
Effect of modification (function):
enzymatic activity, induced, molecular association, regulation
|
|
Modification regulates interactions with:
|
|
Interacting molecule
|
Interacting domains
|
Effect
|
Consequences (function)
|
Consequences (process)
|
Detection assays
|
|
14-3-3 theta (human)
|
|
Induces
|
|
|
co-immunoprecipitation, pull-down assay
|
|
14-3-3 eta (human)
|
|
Induces
|
|
|
co-immunoprecipitation
|
|
|
Comments:
activates PDK1 and promotes 14-3-3 binding, which inhibits PDK1 activity
|
