Curated Information
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Curated Information Page
PubMed Id: 12177059 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Sato S, Fujita N, Tsuruo T (2002) Regulation of kinase activity of 3-phosphoinositide-dependent protein kinase-1 by binding to 14-3-3. J Biol Chem 277, 39360-7 12177059
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S241-p - PDK1 (human)
Orthologous residues
PDK1 (human): S241‑p, PDK1 iso4 (human): S114‑p, PDK1 (mouse): S244‑p, PDK1 (rat): S244‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Disease tissue studied:  fibrosarcoma of soft tissue
 Relevant cell lines - cell types - tissues:  293 (epithelial), COS (fibroblast), HT1080 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, monkey
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PDK1 (human)
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced, molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 eta (human) Induces co-immunoprecipitation
14-3-3 theta (human) Induces pull-down assay, co-immunoprecipitation
 Comments:  activates PDK1 and promotes 14-3-3 binding, which inhibits PDK1 activity


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