Curated Information
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Curated Information Page
PubMed Id: 22865920 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Guise AJ, et al. (2012) Aurora B-dependent regulation of class IIa histone deacetylases by mitotic nuclear localization signal phosphorylation. Mol Cell Proteomics 11, 1220-9 22865920
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S265-p - HDAC4 (human)
Orthologous residues
HDAC4 (human): S265‑p, HDAC4 (mouse): S264‑p, HDAC4 (rat): S264‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), CEM (T lymphocyte), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hesperadin decrease

S278-p - HDAC5 (human)
Orthologous residues
HDAC5 (human): S278‑p, HDAC5 (mouse): S269‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), CEM (T lymphocyte), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE AurB (human) microscopy-colocalization, pharmacological inhibitor of upstream enzyme, siRNA inhibition of enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
siRNA decrease
nocodazole increase
hesperadin nocodazole inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 epsilon (human) Disrupts co-immunoprecipitation
 Comments:  reduced NCoR complex ( NCOR1, TBL1X, TBL1XR1) binding in mitotic cells allows for increased phosphorylation of this site by AurB

S239-p - HDAC9 (human)
Orthologous residues
HDAC9 (human): S239‑p, HDAC9 iso5 (human): S239‑p, HDAC9 iso6 (human): , HDAC9 (mouse): S239‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), CEM (T lymphocyte), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hesperadin decrease


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