Curated Information
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Curated Information Page
PubMed Id: 20417104 
Michael M, Vehlow A, Navarro C, Krause M (2010) c-Abl, Lamellipodin, and Ena/VASP proteins cooperate in dorsal ruffling of fibroblasts and axonal morphogenesis. Curr Biol 20, 783-91 20417104
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y426-p - RAPH1 (human)
Orthologous residues
RAPH1 (human): Y426‑p, RAPH1 (mouse): Y479‑p, RAPH1 iso4 (mouse): Y427‑p, RAPH1 (rat): Y427‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Abl (mouse)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase
Downstream Regulation
 Comments:  regulate dorsal ruffling

Y456-p - RAPH1 (human)
Orthologous residues
RAPH1 (human): Y456‑p, RAPH1 (mouse): Y509‑p, RAPH1 iso4 (mouse): Y457‑p, RAPH1 (rat): Y457‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Abl (mouse)
Downstream Regulation
 Comments:  regulate dorsal ruffling

Y513-p - RAPH1 (human)
Orthologous residues
RAPH1 (human): Y513‑p, RAPH1 (mouse): Y566‑p, RAPH1 iso4 (mouse): Y514‑p, RAPH1 (rat): Y514‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Abl (mouse)
Downstream Regulation
 Comments:  regulate dorsal ruffling

Y1226-p - RAPH1 (human)
Orthologous residues
RAPH1 (human): Y1226‑p, RAPH1 (mouse): , RAPH1 iso4 (mouse): Y1242‑p, RAPH1 (rat): Y1242‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Abl (mouse)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase
Downstream Regulation
 Comments:  regulate dorsal ruffling

Y479-p - RAPH1 (mouse)
Orthologous residues
RAPH1 (human): Y426‑p, RAPH1 (mouse): Y479‑p, RAPH1 iso4 (mouse): Y427‑p, RAPH1 (rat): Y427‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Abl (mouse) phospho-antibody, transfection of inactive enzyme
 Comments:  interaction mediated by Abl SH2 domain

Y1242-p - RAPH1 iso4 (mouse)
Orthologous residues
RAPH1 (human): Y1226‑p, RAPH1 (mouse): , RAPH1 iso4 (mouse): Y1242‑p, RAPH1 (rat): Y1242‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Abl (mouse) phospho-antibody, transfection of inactive enzyme
 Comments:  interaction mediated by Abl SH2 domain


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