Curated Information
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Curated Information Page
PubMed Id: 22863012 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Qiang L, et al. (2012) Brown Remodeling of White Adipose Tissue by SirT1-Dependent Deacetylation of PparĪ³. Cell 150, 620-32 22863012
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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K268-ac - PPAR-gamma (mouse)
Orthologous residues
PPAR‑gamma (human): K268‑ac, PPAR‑gamma iso3 (human): , PPAR‑gamma (mouse): K268‑ac, PPAR‑gamma iso2 (mouse): K238‑ac, PPAR‑gamma (rat): K268‑ac
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3-L1 (fibroblast), Swiss 3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
DEACETYLASE SIRT1 (human) phosphopeptide analysis, co-immunoprecipitation
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
N-CoR1 (mouse) Induces co-immunoprecipitation
 Comments:  deacetylation mediates TZD-induced browning of white adipocytes enhancing expression of BAT genes; PPAR-gamma acetylation and phosphorylation promote adipokine production.

S273-p - PPAR-gamma (mouse)
Orthologous residues
PPAR‑gamma (human): S273‑p, PPAR‑gamma iso3 (human): , PPAR‑gamma (mouse): S273‑p, PPAR‑gamma iso2 (mouse): S243‑p, PPAR‑gamma (rat): S273‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3-L1 (fibroblast), Swiss 3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Downstream Regulation
 Comments:  PPAR-gamma acetylation and phosphorylation promote adipokine production.

K293-ac - PPAR-gamma (mouse)
Orthologous residues
PPAR‑gamma (human): K293‑ac, PPAR‑gamma iso3 (human): , PPAR‑gamma (mouse): K293‑ac, PPAR‑gamma iso2 (mouse): K263‑ac, PPAR‑gamma (rat): K293‑ac
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3-L1 (fibroblast), Swiss 3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
DEACETYLASE SIRT1 (human) phosphopeptide analysis, co-immunoprecipitation
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PRDM1 (mouse) Induces co-immunoprecipitation
N-CoR1 (mouse) Induces co-immunoprecipitation
 Comments:  deacetylation mediates TZD-induced browning of white adipocytes enhancing expression of BAT genes; PPAR-gamma acetylation and phosphorylation promote adipokine production.


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