Curated Information
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Curated Information Page
PubMed Id: 22885697 
Qu Y, et al. (2012) Phosphorylation of NLRC4 is critical for inflammasome activation. Nature 490, 539-42 22885697
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S533-p - NLRC4 (mouse)
Orthologous residues
NLRC4 (human): S533‑p, NLRC4 (mouse): S533‑p, NLRC4 (rat): S533‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  macrophage-bone marrow
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCD (mouse)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKCD (mouse) pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
bacterial infection increase infection with S. typhimurium and L.pneumophila
LPS no change compared to control
staurosporine bacterial infection inhibit treatment-induced increase
K252a bacterial infection inhibit treatment-induced increase
rottlerin bacterial infection inhibit treatment-induced increase
Downstream Regulation
 Comments:  citical for NLRC4 inflammasome activation

Y311-p - PKCD (mouse)
Orthologous residues
PKCD (human): Y313‑p, PKCD (mouse): Y311‑p, PKCD iso2 (mouse): Y311‑p, PKCD (rat): Y311‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  macrophage-bone marrow
 Cellular systems studied:  primary cultured cells
 Species studied:  dog
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
bacterial infection increase S. typhimurium infection


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