Curated Information
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Curated Information Page
PubMed Id: 17045821 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Shi X, et al. (2006) CD44 is the signaling component of the macrophage migration inhibitory factor-CD74 receptor complex. Immunity 25, 595-606 17045821
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T202-p - ERK1 (human)
Orthologous residues
ERK1 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast), macrophage, MEF (fibroblast)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  monkey, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
MIF no change compared to control COS cells
MIF CD74 (human) no change compared to control
MIF CD44 (human) no change compared to control
MIF CD44 (human), CD74 (human) increase
siRNA Src (human) CD44 (human), CD74 (human) inhibit treatment-induced increase
PP2 CD44 (human), CD74 (human) inhibit treatment-induced increase

Y204-p - ERK1 (human)
Orthologous residues
ERK1 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast), macrophage, MEF (fibroblast)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  monkey, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
MIF no change compared to control COS cells
MIF CD74 (human) no change compared to control
MIF CD44 (human) no change compared to control
MIF CD44 (human), CD74 (human) increase
siRNA Src (human) CD44 (human), CD74 (human) inhibit treatment-induced increase
PP2 CD44 (human), CD74 (human) inhibit treatment-induced increase

T185-p - ERK2 (human)
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast), macrophage, MEF (fibroblast)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  monkey, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
MIF no change compared to control COS cells
MIF CD74 (human) no change compared to control
MIF CD44 (human) no change compared to control
MIF CD44 (human), CD74 (human) increase
siRNA Src (human) CD44 (human), CD74 (human) inhibit treatment-induced increase
PP2 CD44 (human), CD74 (human) inhibit treatment-induced increase

Y187-p - ERK2 (human)
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast), macrophage, MEF (fibroblast)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  monkey, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
MIF no change compared to control COS cells
MIF CD74 (human) no change compared to control
MIF CD44 (human) no change compared to control
MIF CD44 (human), CD74 (human) increase
siRNA Src (human) CD44 (human), CD74 (human) inhibit treatment-induced increase
PP2 CD44 (human), CD74 (human) inhibit treatment-induced increase

S15-p - p53 (human)
Orthologous residues
p53 (human): S15‑p, p53 (mouse): S15‑p, p53 iso2 (mouse): S18‑p, p53 (rat): S15‑p, p53 (rabbit): S15‑p, p53 (monkey): S15‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast), macrophage, MEF (fibroblast)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  monkey, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
MIF no change compared to control COS
MIF CD74 (human) no change compared to control
MIF CD44 (human) no change compared to control
MIF CD44 (human), CD74 (human) decrease

Y419-p - Src (human)
Orthologous residues
Src (human): Y419‑p, Src (mouse): Y424‑p, Src iso2 (mouse): Y418‑p, Src (rat): Y419‑p, Src (chicken): Y416‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast), macrophage, MEF (fibroblast)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  monkey, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
MIF no change compared to control COS cells
MIF CD74 (human) no change compared to control
MIF CD44 (human) no change compared to control
MIF CD44 (human), CD74 (human) increase
siRNA Src (human) CD44 (human), CD74 (human) inhibit treatment-induced increase
PP2 CD44 (human), CD74 (human) inhibit treatment-induced increase

T203-p - ERK1 (mouse)
Orthologous residues
ERK1 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast), macrophage, MEF (fibroblast)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  monkey, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
MIF increase
MIF CD74 (mouse) inhibit treatment-induced increase homozygous knockout
MIF CD44 (mouse) inhibit treatment-induced increase homozygous knockout
PP2 MIF inhibit treatment-induced increase

Y205-p - ERK1 (mouse)
Orthologous residues
ERK1 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast), macrophage, MEF (fibroblast)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  monkey, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
MIF increase
MIF CD74 (mouse) inhibit treatment-induced increase homozygous knockout
MIF CD44 (mouse) inhibit treatment-induced increase homozygous knockout
PP2 MIF inhibit treatment-induced increase

T183-p - ERK2 (mouse)
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast), macrophage, MEF (fibroblast)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  monkey, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
MIF increase
MIF CD74 (mouse) inhibit treatment-induced increase homozygous knockout
MIF CD44 (mouse) inhibit treatment-induced increase homozygous knockout
PP2 MIF inhibit treatment-induced increase

Y185-p - ERK2 (mouse)
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast), macrophage, MEF (fibroblast)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  monkey, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
MIF increase
MIF CD74 (mouse) inhibit treatment-induced increase homozygous knockout
MIF CD44 (mouse) inhibit treatment-induced increase homozygous knockout
PP2 MIF inhibit treatment-induced increase

S15-p - p53 (mouse)
Orthologous residues
p53 (human): S15‑p, p53 (mouse): S15‑p, p53 iso2 (mouse): S18‑p, p53 (rat): S15‑p, p53 (rabbit): S15‑p, p53 (monkey): S15‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast), macrophage, MEF (fibroblast)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  monkey, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
MIF decrease
MIF CD74 (mouse) augment treatment-induced decrease homozygous knockout
MIF CD44 (mouse) augment treatment-induced decrease homozygous knockout
CD74 (mouse) decrease homozygous knockout
CD44 (mouse) decrease homozygous knockout

Y424-p - Src (mouse)
Orthologous residues
Src (human): Y419‑p, Src (mouse): Y424‑p, Src iso2 (mouse): Y418‑p, Src (rat): Y419‑p, Src (chicken): Y416‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast), macrophage, MEF (fibroblast)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  monkey, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
MIF increase
MIF CD74 (mouse) inhibit treatment-induced increase homozygous knockout
MIF CD44 (mouse) inhibit treatment-induced increase homozygous knockout
PP2 MIF inhibit treatment-induced increase


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