Curated Information
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Curated Information Page
PubMed Id: 16818236 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Lu C, et al. (2006) Cell apoptosis: requirement of H2AX in DNA ladder formation, but not for the activation of caspase-3. Mol Cell 23, 121-32 16818236
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T183-p - JNK1 iso2 (human) ▲
Orthologous residues
JNK1 (human): T183‑p, JNK1 iso2 (human): T183‑p, JNK1 iso3 (human): T183‑p, JNK1 (mouse): T183‑p, JNK1 (rat): T183‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  JB6 RT101 (epidermal)
 Cellular systems studied:  cell lines
 Species studied:  mouse

Y185-p - JNK1 iso2 (human) ▲
Orthologous residues
JNK1 (human): Y185‑p, JNK1 iso2 (human): Y185‑p, JNK1 iso3 (human): Y185‑p, JNK1 (mouse): Y185‑p, JNK1 (rat): Y185‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  JB6 RT101 (epidermal)
 Cellular systems studied:  cell lines
 Species studied:  mouse

T53-p - ATF-2 (mouse) ▲
Orthologous residues
ATF‑2 (human): T71‑p, ATF‑2 (mouse): T53‑p, ATF‑2 (rat): T53‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  JB6 RT101 (epidermal)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
UV p38-beta (mouse) inhibit treatment-induced increase dominant negative

T55-p - ATF-2 (mouse) ▲
Orthologous residues
ATF‑2 (human): T73‑p, ATF‑2 (mouse): T55‑p, ATF‑2 (rat): T55‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  JB6 RT101 (epidermal)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
UV p38-beta (mouse) inhibit treatment-induced increase dominant negative

T203-p - ERK1 (mouse) ▲
Orthologous residues
ERK1 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  JB6 RT101 (epidermal)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
UV ERK2 (mouse) inhibit treatment-induced increase dominant negative

Y205-p - ERK1 (mouse) ▲
Orthologous residues
ERK1 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  JB6 RT101 (epidermal)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
UV ERK2 (mouse) inhibit treatment-induced increase dominant negative

T183-p - ERK2 (mouse) ▲
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  JB6 RT101 (epidermal)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
UV ERK2 (mouse) inhibit treatment-induced increase dominant negative

Y185-p - ERK2 (mouse) ▲
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  JB6 RT101 (epidermal)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
UV ERK2 (mouse) inhibit treatment-induced increase dominant negative

S140-p - H2AX (mouse) ▲
Orthologous residues
H2AX (human): S140‑p, H2AX (mouse): S140‑p, H2AX (rat): S140‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), JB6 RT101 (epidermal), MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE JNK1 (mouse)
KINASE JNK2 (mouse)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JNK2 (mouse) genetic knockout/knockin of upstream enzyme, microscopy-colocalization, pharmacological activator of upstream enzyme, co-immunoprecipitation, pharmacological inhibitor of upstream enzyme, phospho-antibody
KINASE JNK1 (mouse) transfection of dominant-negative enzyme, microscopy-colocalization, pharmacological activator of upstream enzyme, siRNA inhibition of enzyme, co-immunoprecipitation, pharmacological inhibitor of upstream enzyme, phospho-antibody
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
SP600125 UV inhibit treatment-induced increase
PD98059 UV no effect upon treatment-induced increase
SB202190 UV no effect upon treatment-induced increase
UV ERK2 (mouse) no effect upon treatment-induced increase dominant negative
UV p38-beta (mouse) no effect upon treatment-induced increase dominant negative
UV JNK1 (mouse) inhibit treatment-induced increase dominant negative
UV JNK2 (mouse) inhibit treatment-induced increase homozygous knockout
siRNA JNK1 (mouse) JNK2 (mouse) augment treatment-induced decrease
Downstream Regulation
 Effect of modification (function):  activation
 Effect of modification (process):  apoptosis, induced

S63-p - Jun (mouse) ▲
Orthologous residues
Jun (human): S63‑p, Jun (mouse): S63‑p, Jun (rat): S63‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  JB6 RT101 (epidermal)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
JNK1 (mouse) inhibit treatment-induced increase dominant negative


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