Curated Information
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Curated Information Page
PubMed Id: 22306293 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Gao X, et al. (2012) Pyruvate kinase M2 regulates gene transcription by acting as a protein kinase. Mol Cell 45, 598-609 22306293
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Y705-p - STAT3 (human)
Orthologous residues
STAT3 (human): Y705‑p, STAT3 iso2 (human): Y704‑p, STAT3 (mouse): Y705‑p, STAT3 iso2 (mouse): Y705‑p, STAT3 iso3 (mouse): Y704‑p, STAT3 (rat): Y705‑p, STAT3 (cow): Y705‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
 Disease tissue studied:  colorectal cancer, colorectal carcinoma
 Relevant cell lines - cell types - tissues:  SW620 (intestinal)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKM2 (human)
 Comments:  in the presence of PEP (not ATP) as phosphate donor, Nuclear PKM2 (dimer) has higher activity than cytoplasmic (tetramer).
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKM2 (human) siRNA inhibition of enzyme, transfection of wild-type enzyme
Downstream Regulation
 Effect of modification (process):  cell growth, induced, transcription, induced
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
DNA Induces transcription, induced EMSA
 Comments:  PKM2 R399E mutant has higher activity, increasing STAT3 phosphorylation and inducing cell proliferation. Upregulates MEK5 transcription.


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