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Orthologous residues
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STAT3 (human): Y705‑p, STAT3 iso2 (human): Y704‑p, STAT3 iso3 (human): Y705‑p, STAT3 (mouse): Y705‑p, STAT3 iso2 (mouse): Y705‑p, STAT3 iso3 (mouse): Y704‑p, STAT3 (rat): Y705‑p, STAT3 (cow): Y705‑p
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Characterization
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Methods used to characterize site in vivo:
immunoprecipitation, phospho-antibody, western blotting
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Disease tissue studied:
colorectal cancer, colorectal carcinoma
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Relevant cell lines - cell types - tissues:
SW620 (intestinal)
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Cellular systems studied:
cell lines
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Species studied:
human
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Enzymes shown to modify site in vitro:
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|
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Comments:
in the presence of PEP (not ATP) as phosphate donor, Nuclear PKM2 (dimer) has higher activity than cytoplasmic (tetramer).
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Upstream Regulation
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Potential in vivo enzymes for site:
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Type
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Enzyme
|
Evidence
|
Notes
|
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KINASE
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PKM2 (human)
|
transfection of wild-type enzyme, siRNA inhibition of enzyme
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Downstream Regulation
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Effect of modification (process):
cell growth, induced, transcription, induced
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Modification regulates interactions with:
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|
Interacting molecule
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Interacting domains
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Effect
|
Consequences (function)
|
Consequences (process)
|
Detection assays
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|
DNA
|
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Induces
|
|
transcription, induced
|
EMSA
|
|
|
Comments:
PKM2 R399E mutant has higher activity, increasing STAT3 phosphorylation and inducing cell proliferation. Upregulates MEK5 transcription.
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