Curated Information
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Curated Information Page
PubMed Id: 16962653 
Jacinto E, et al. (2006) SIN1/MIP1 maintains rictor-mTOR complex integrity and regulates Akt phosphorylation and substrate specificity. Cell 127, 125-37 16962653
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
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T36-p - 4E-BP1 (mouse)
Orthologous residues
4E‑BP1 (human): T37‑p, 4E‑BP1 (mouse): T36‑p, 4E‑BP1 (rat): T36‑p, 4E‑BP1 (fruit fly): T37‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum starvation decrease
serum increase
serum Sin1 (mouse) no effect upon treatment-induced increase homozygous knockout
insulin increase
insulin Sin1 (mouse) no effect upon treatment-induced increase homozygous knockout

T45-p - 4E-BP1 (mouse)
Orthologous residues
4E‑BP1 (human): T46‑p, 4E‑BP1 (mouse): T45‑p, 4E‑BP1 (rat): T45‑p, 4E‑BP1 (fruit fly): T46‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum starvation decrease
serum increase
serum Sin1 (mouse) no effect upon treatment-induced increase homozygous knockout
insulin increase
insulin Sin1 (mouse) no effect upon treatment-induced increase homozygous knockout

T308-p - Akt1 (mouse)
Orthologous residues
Akt1 (human): T308‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum starvation decrease
serum increase
serum Sin1 (mouse) no effect upon treatment-induced increase homozygous knockout
insulin increase
insulin Sin1 (mouse) no effect upon treatment-induced increase homozygous knockout
PDGF increase
PDGF Sin1 (mouse) no effect upon treatment-induced increase homozygous knockout
EGF increase
EGF Sin1 (mouse) no effect upon treatment-induced increase homozygous knockout
FGF2 increase
FGF2 Sin1 (mouse) no effect upon treatment-induced increase homozygous knockout

S473-p - Akt1 (mouse)
Orthologous residues
Akt1 (human): S473‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum increase
serum Sin1 (mouse) inhibit treatment-induced increase homozygous knockout
insulin increase
insulin Sin1 (mouse) inhibit treatment-induced increase homozygous knockout
PDGF increase
PDGF Sin1 (mouse) inhibit treatment-induced increase homozygous knockout
EGF increase
EGF Sin1 (mouse) inhibit treatment-induced increase homozygous knockout
FGF2 no change compared to control

T24-p - FOXO1A (mouse)
Orthologous residues
FOXO1A (human): T24‑p, FOXO1A (mouse): T24‑p, FOXO1A (rat): T24‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum increase
serum Sin1 (mouse) inhibit treatment-induced increase homozygous knockout
insulin increase
insulin Sin1 (mouse) inhibit treatment-induced increase homozygous knockout

T32-p - FOXO3A (mouse)
Orthologous residues
FOXO3A (human): T32‑p, FOXO3A (mouse): T32‑p, FOXO3A (rat): T32‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum increase
serum Sin1 (mouse) inhibit treatment-induced increase homozygous knockout
insulin increase
insulin Sin1 (mouse) inhibit treatment-induced increase homozygous knockout

S21-p - GSK3A (mouse)
Orthologous residues
GSK3A (human): S21‑p, GSK3A (mouse): S21‑p, GSK3A (rat): S21‑p, GSK3A (cow): S21‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum starvation decrease
serum increase
serum Sin1 (mouse) no effect upon treatment-induced increase homozygous knockout
insulin increase
insulin Sin1 (mouse) no effect upon treatment-induced increase homozygous knockout

S9-p - GSK3B (mouse)
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S3‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum starvation decrease
serum increase
serum Sin1 (mouse) no effect upon treatment-induced increase homozygous knockout
insulin increase
insulin Sin1 (mouse) no effect upon treatment-induced increase homozygous knockout

T412-p - p70S6K (mouse)
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum starvation decrease
serum increase
serum Sin1 (mouse) no effect upon treatment-induced increase homozygous knockout
insulin increase
insulin Sin1 (mouse) no effect upon treatment-induced increase homozygous knockout

S939-p - TSC2 (mouse)
Orthologous residues
TSC2 (human): S939‑p, TSC2 iso3 (human): S939‑p, TSC2 iso4 (human): S939‑p, TSC2 (mouse): S939‑p, TSC2 iso6 (mouse): S939‑p, TSC2 (rat): S939‑p, TSC2 iso2 (rat): S939‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum starvation decrease
serum increase
serum Sin1 (mouse) no effect upon treatment-induced increase homozygous knockout
insulin increase
insulin Sin1 (mouse) no effect upon treatment-induced increase homozygous knockout

T1465-p - TSC2 (mouse)
Orthologous residues
TSC2 (human): T1462‑p, TSC2 iso3 (human): T1418‑p, TSC2 iso4 (human): T1439‑p, TSC2 (mouse): T1465‑p, TSC2 iso6 (mouse): T1443‑p, TSC2 (rat): T1466‑p, TSC2 iso2 (rat): T1423‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum starvation decrease
serum increase
serum Sin1 (mouse) no effect upon treatment-induced increase homozygous knockout
insulin increase
insulin Sin1 (mouse) no effect upon treatment-induced increase homozygous knockout


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