Curated Information
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Curated Information Page
PubMed Id: 22629392 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Wang S, et al. (2012) Extensive Crosstalk between O-GlcNAcylation and Phosphorylation Regulates Akt Signaling. PLoS One 7, e37427 22629392
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S126-gl - Akt1 (human)
Orthologous residues
Akt1 (human): S126‑gl, Akt1 (mouse): S126‑gl, Akt1 (rat): S126‑gl, Akt1 (fruit fly): A238‑gl, Akt1 (cow): G126‑gl
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Disease tissue studied:  breast cancer
 Relevant cell lines - cell types - tissues:  MCF-7 (breast cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PUGNAc increase

S129-gl - Akt1 (human)
Orthologous residues
Akt1 (human): S129‑gl, Akt1 (mouse): S129‑gl, Akt1 (rat): S129‑gl, Akt1 (fruit fly): E241‑gl, Akt1 (cow): S129‑gl
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Disease tissue studied:  breast cancer
 Relevant cell lines - cell types - tissues:  MCF-7 (breast cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PUGNAc increase

T305-gl - Akt1 (human)
Orthologous residues
Akt1 (human): T305‑gl, Akt1 (mouse): T305‑gl, Akt1 (rat): T305‑gl, Akt1 (fruit fly): T420‑gl, Akt1 (cow): T305‑gl
Characterization
 Methods used to characterize site in vivo mass spectrometry, modification-specific antibody, mutation of modification site, western blotting
 Disease tissue studied:  breast cancer
 Relevant cell lines - cell types - tissues:  COS (fibroblast), MCF-7 (breast cell)
 Cellular systems studied:  cell lines
 Species studied:  monkey
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PUGNAc increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, inhibited, phosphorylation
 Comments:  suppresses Akt phosphorylation at T308 by disrupting its interaction with PDK1

T308-p - Akt1 (human)
Orthologous residues
Akt1 (human): T308‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  breast cancer
 Relevant cell lines - cell types - tissues:  MCF-7 (breast cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PUGNAc decrease
Akt1 (human) decrease Akt T305A and T312A glycosylation mutants

T312-gl - Akt1 (human)
Orthologous residues
Akt1 (human): T312‑gl, Akt1 (mouse): T312‑gl, Akt1 (rat): T312‑gl, Akt1 (fruit fly): T427‑gl, Akt1 (cow): T312‑gl
Characterization
 Methods used to characterize site in vivo mass spectrometry, modification-specific antibody, mutation of modification site, western blotting
 Disease tissue studied:  breast cancer
 Relevant cell lines - cell types - tissues:  COS (fibroblast), MCF-7 (breast cell)
 Cellular systems studied:  cell lines
 Species studied:  monkey
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PUGNAc increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, inhibited, phosphorylation
 Comments:  suppresses Akt phosphorylation at T308 by disrupting its interaction with PDK1

S473-p - Akt1 (human)
Orthologous residues
Akt1 (human): S473‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  breast cancer
 Relevant cell lines - cell types - tissues:  MCF-7 (breast cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PUGNAc decrease


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