Curated Information
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PubMed Id: 16982679 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Limesand KH, Schwertfeger KL, Anderson SM (2006) MDM2 is required for suppression of apoptosis by activated Akt1 in salivary acinar cells. Mol Cell Biol 26, 8840-56 16982679
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S345-p - Chk1 (mouse) ▲
Orthologous residues
Chk1 (human): S345‑p, Chk1 (mouse): S345‑p, Chk1 (rat): S345‑p, Chk1 (chicken): S345‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  parotid acinar-salivary gland, parotid acinar-salivary gland [Akt1 (mouse)]
 Cellular systems studied:  primary cells, tissue
 Species studied:  mouse
 Comments:  +/- myr-Akt1
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
etoposide Akt1 (mouse) no change compared to control constitutively active
siRNA etoposide MDM2 (mouse) Akt1 (mouse) increase
siRNA MDM2 (mouse) increase in constitutively active Akt1-expressing cells

S21-p - GSK3A (mouse) ▲
Orthologous residues
GSK3A (human): S21‑p, GSK3A (mouse): S21‑p, GSK3A (rat): S21‑p, GSK3A (cow): S21‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  parotid acinar-salivary gland, parotid acinar-salivary gland [Akt1 (mouse)]
 Cellular systems studied:  primary cells, tissue
 Species studied:  mouse
 Comments:  +/- myr-Akt1
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Akt1 (mouse) increase constitutively active

S9-p - GSK3B (mouse) ▲
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S3‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  parotid acinar-salivary gland, parotid acinar-salivary gland [Akt1 (mouse)]
 Cellular systems studied:  primary cells, tissue
 Species studied:  mouse
 Comments:  +/- myr-Akt1
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Akt1 (mouse) increase constitutively active

S163-p - MDM2 (mouse) ▲
Orthologous residues
MDM2 (human): S166‑p, MDM2 iso12 (human): ‑, MDM2 (mouse): S163‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  parotid acinar-salivary gland, parotid acinar-salivary gland [Akt1 (mouse)]
 Cellular systems studied:  primary cells, tissue
 Species studied:  mouse
 Comments:  +/- myr-Akt1
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Akt1 (mouse) increase constitutively active
Akt1 (mouse) decrease dominant negative

S15-p - p53 (mouse) ▲
Orthologous residues
p53 (human): S15‑p, p53 (mouse): S15‑p, p53 iso2 (mouse): S18‑p, p53 (rat): S15‑p, p53 (rabbit): S15‑p, p53 (monkey): S15‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  parotid acinar-salivary gland, parotid acinar-salivary gland [Akt1 (mouse)]
 Cellular systems studied:  primary cells, tissue
 Species studied:  mouse
 Comments:  +/- myr-Akt1
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase
ionizing radiation Akt1 (mouse) inhibit treatment-induced increase constitutively active
etoposide increase
etoposide Akt1 (mouse) inhibit treatment-induced increase constitutively active
siRNA MDM2 (human) Akt1 (mouse) inhibit treatment-induced decrease


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